Detailed information of TA system TA238036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 245526..246176
• Replicon: chromosome
• Accession: NZ_CP093300
• Organism: Alkalihalobacillus sp. LMS39

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MM271_RS01345
• Protein ID: WP_026675763.1
• Coordinates: 245826..246176 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: MM271_RS01340
• Protein ID: WP_279390783.1
• Coordinates: 245526..245822 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MM271_RS01320 (MM271_01320)	241117..241869	-	753	WP_243530657.1	rhomboid family intramembrane serine protease	-
MM271_RS01325 (MM271_01325)	241977..242330	+	354	WP_243530660.1	holo-ACP synthase	-
MM271_RS01330 (MM271_01330)	242878..243903	+	1026	WP_243530663.1	outer membrane lipoprotein carrier protein LolA	-
MM271_RS01335 (MM271_01335)	244255..245430	+	1176	WP_243530665.1	alanine racemase	-
MM271_RS01340 (MM271_01340)	245526..245822	+	297	WP_279390783.1	antitoxin	Antitoxin
MM271_RS01345 (MM271_01345)	245826..246176	+	351	WP_026675763.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MM271_RS01350 (MM271_01350)	246323..247141	+	819	WP_243530669.1	RsbT co-antagonist protein RsbRA	-
MM271_RS01355 (MM271_01355)	247157..247513	+	357	WP_026675765.1	STAS domain-containing protein	-
MM271_RS01360 (MM271_01360)	247518..247928	+	411	WP_026675766.1	anti-sigma regulatory factor	-
MM271_RS01365 (MM271_01365)	247940..248965	+	1026	WP_279390784.1	PP2C family protein-serine/threonine phosphatase	-
MM271_RS01370 (MM271_01370)	249020..249361	+	342	WP_243530671.1	STAS domain-containing protein	-
MM271_RS01375 (MM271_01375)	249358..249840	+	483	WP_243530674.1	anti-sigma B factor RsbW	-
MM271_RS01380 (MM271_01380)	249803..250588	+	786	WP_243530676.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12961.98 Da
• Isoelectric Point: 6.4662

>T238036 WP_026675763.1 NZ_CP093300:245826-246176 [Alkalihalobacillus sp. LMS39]
MIVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDDMMSRVNDALQISLGLIDF

Nucleotide

• Length: 351 bp

>T238036 NZ_LR890189:c4675785-4675501 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11407.14 Da
• Isoelectric Point: 7.2033

>AT238036 WP_279390783.1 NZ_CP093300:245526-245822 [Alkalihalobacillus sp. LMS39]
MLGGFFVSENSTKRIMVNLPQHILNEVDGVIKQENVNRSEFIYQATKMYLRERKKRKIRETMQQGYMEMAKINLNIASEA
FLAEEEAEHTLDRLVSGV

Nucleotide

• Length: 297 bp

>AT238036 NZ_LR890189:c4676017-4675775 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA