Detailed information of TA system TA238035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2637606..2638234
• Replicon: chromosome
• Accession: NZ_CP093299
• Organism: Salipaludibacillus sp. LMS25

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MM221_RS12340
• Protein ID: WP_078578026.1
• Coordinates: 2637884..2638234 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MM221_RS12335
• Protein ID: WP_255238211.1
• Coordinates: 2637606..2637878 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MM221_RS12315 (MM221_12200)	2632637..2633005	+	369	WP_255238210.1	holo-ACP synthase	-
MM221_RS12320 (MM221_12205)	2633079..2634608	+	1530	WP_255234616.1	NAD(P)H-hydrate dehydratase	-
MM221_RS12325 (MM221_12210)	2634661..2635695	+	1035	WP_255234617.1	outer membrane lipoprotein carrier protein LolA	-
MM221_RS12330 (MM221_12215)	2636112..2637287	+	1176	WP_255234618.1	alanine racemase	-
MM221_RS12335 (MM221_12220)	2637606..2637878	+	273	WP_255238211.1	antitoxin	Antitoxin
MM221_RS12340 (MM221_12225)	2637884..2638234	+	351	WP_078578026.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MM221_RS12345 (MM221_12230)	2638487..2639311	+	825	WP_255234619.1	RsbT co-antagonist protein RsbRA	-
MM221_RS12350 (MM221_12235)	2639326..2639682	+	357	WP_255234620.1	STAS domain-containing protein	-
MM221_RS12355 (MM221_12240)	2639686..2640087	+	402	WP_255234621.1	anti-sigma regulatory factor	-
MM221_RS12360 (MM221_12245)	2640123..2641115	+	993	WP_255238212.1	PP2C family protein-serine/threonine phosphatase	-
MM221_RS12365 (MM221_12250)	2641181..2641510	+	330	WP_255234622.1	STAS domain-containing protein	-
MM221_RS12370 (MM221_12255)	2641512..2642006	+	495	WP_255234623.1	anti-sigma B factor RsbW	-
MM221_RS12375 (MM221_12260)	2641966..2642775	+	810	WP_255234624.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12932.00 Da
• Isoelectric Point: 6.4662

>T238035 WP_078578026.1 NZ_CP093299:2637884-2638234 [Salipaludibacillus sp. LMS25]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEINAKRYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDDMMLKVNDALNISIGLIDF

Nucleotide

• Length: 351 bp

>T238035 NZ_LR890189:c4572107-4571574 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCAGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10297.75 Da
• Isoelectric Point: 4.4232

>AT238035 WP_255238211.1 NZ_CP093299:2637606-2637878 [Salipaludibacillus sp. LMS25]
MENTKKIMVNLPQHLVNELDALTEGEGINRCDVIQRATRIYVQEQKKLQIRETMEQGYMEMAKINLNIATESFLAEEEAE
STVDRLVSGV

Nucleotide

• Length: 273 bp

>AT238035 NZ_LR890189:c4572384-4572118 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA