Detailed information of TA system TA238032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 4225135..4226113
• Replicon: chromosome
• Accession: NZ_CP093291
• Organism: Bacillus mycoides strain DSM 2048

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: R8N1U0
• Locus tag: MN093_RS21955
• Protein ID: WP_002032139.1
• Coordinates: 4225135..4225875 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: MN093_RS21960
• Protein ID: WP_000588712.1
• Coordinates: 4225988..4226113 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN093_RS21925 (MN093_21925)	4220235..4220996	+	762	WP_131246197.1	ABC transporter ATP-binding protein	-
MN093_RS21930 (MN093_21930)	4221103..4221813	+	711	WP_044439620.1	class I SAM-dependent methyltransferase	-
MN093_RS21935 (MN093_21935)	4221932..4222345	+	414	WP_002012925.1	VOC family protein	-
MN093_RS21940 (MN093_21940)	4222522..4223154	-	633	WP_131246198.1	type 1 glutamine amidotransferase family protein	-
MN093_RS21945 (MN093_21945)	4223428..4223904	+	477	WP_002017049.1	MarR family winged helix-turn-helix transcriptional regulator	-
MN093_RS21950 (MN093_21950)	4224058..4224795	+	738	WP_002032137.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
MN093_RS21955 (MN093_21955)	4225135..4225875	+	741	WP_002032139.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MN093_RS21960 (MN093_21960)	4225988..4226113	+	126	WP_000588712.1	hypothetical protein	Antitoxin
MN093_RS21965 (MN093_21965)	4226189..4226365	+	177	WP_002017052.1	stage II sporulation protein SB	-
MN093_RS21970 (MN093_21970)	4226385..4226774	-	390	WP_241875896.1	YxeA family protein	-
MN093_RS21975 (MN093_21975)	4227026..4228495	+	1470	WP_016102002.1	aminoacyl-histidine dipeptidase	-
MN093_RS21980 (MN093_21980)	4228855..4229004	+	150	WP_002069316.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28482.11 Da
• Isoelectric Point: 8.3034

>T238032 WP_002032139.1 NZ_CP093291:4225135-4225875 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWRNEELYEEKKQRIRKTWYGLFITSVTVYFTIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPTAFGTMEWHTEEEYTKSLNTFLDSYGEKIGAK
IVVFEAVKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T238032 NZ_LR890189:2371207-2371524 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT238032 WP_000588712.1 NZ_CP093291:4225988-4226113 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT238032 NZ_LR890189:2371600-2371896 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCCGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCAGCCAACCGGTGCAGCCAGAAAA
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Structures

Toxin

• AlphaFold DB: R8N1U0

Antitoxin

• AlphaFold DB: A0A366GQT1