Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2055572..2056214 Replicon chromosome
Accession NZ_CP093291
Organism Bacillus mycoides strain DSM 2048

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag MN093_RS10860 Protein ID WP_000635965.1
Coordinates 2055864..2056214 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag MN093_RS10855 Protein ID WP_000004570.1
Coordinates 2055572..2055859 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MN093_RS10830 (MN093_10830) 2050887..2051849 + 963 WP_131248046.1 UV DNA damage repair endonuclease UvsE -
MN093_RS10835 (MN093_10835) 2051842..2052414 - 573 WP_113735921.1 rhomboid family intramembrane serine protease -
MN093_RS10840 (MN093_10840) 2052507..2052866 + 360 WP_088034763.1 holo-ACP synthase -
MN093_RS10845 (MN093_10845) 2053023..2053973 + 951 WP_002091373.1 outer membrane lipoprotein carrier protein LolA -
MN093_RS10850 (MN093_10850) 2054091..2055260 + 1170 WP_088034765.1 alanine racemase -
MN093_RS10855 (MN093_10855) 2055572..2055859 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
MN093_RS10860 (MN093_10860) 2055864..2056214 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MN093_RS10865 (MN093_10865) 2056283..2058451 + 2169 WP_131248052.1 Tex family protein -
MN093_RS10870 (MN093_10870) 2058509..2058625 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
MN093_RS10875 (MN093_10875) 2058836..2059291 + 456 WP_016095131.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T238031 WP_000635965.1 NZ_CP093291:2055864-2056214 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T238031 NZ_LR890189:1884932-1885034 [Klebsiella pneumoniae]
GCAAGGCGGCTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT238031 WP_000004570.1 NZ_CP093291:2055572-2055859 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT238031 NZ_LR890189:c1885041-1884896 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGCCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References