Detailed information of TA system TA238030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1774315..1775234
• Replicon: chromosome
• Accession: NZ_CP093290
• Organism: Bacillus licheniformis strain DSM 13

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: MN092_RS09025
• Protein ID: WP_003180879.1
• Coordinates: 1774488..1775234 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: T5HT37
• Locus tag: MN092_RS09020
• Protein ID: WP_003180877.1
• Coordinates: 1774315..1774488 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN092_RS08995 (MN092_08995)	1769740..1770837	+	1098	WP_003180867.1	mannonate dehydratase	-
MN092_RS09000 (MN092_09000)	1770813..1771661	+	849	WP_009328728.1	SDR family oxidoreductase	-
MN092_RS09005 (MN092_09005)	1771688..1772701	+	1014	WP_003180872.1	zinc-binding alcohol dehydrogenase family protein	-
MN092_RS09010 (MN092_09010)	1772754..1774025	+	1272	WP_011197811.1	MFS transporter	-
MN092_RS09015 (MN092_09015)	1774088..1774219	-	132	WP_003180875.1	hypothetical protein	-
MN092_RS09020 (MN092_09020)	1774315..1774488	-	174	WP_003180877.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
MN092_RS09025 (MN092_09025)	1774488..1775234	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MN092_RS09030 (MN092_09030)	1775345..1776343	-	999	WP_009328723.1	inorganic phosphate transporter	-
MN092_RS09035 (MN092_09035)	1776356..1776973	-	618	WP_003180884.1	DUF47 domain-containing protein	-
MN092_RS09040 (MN092_09040)	1777305..1779062	+	1758	WP_061565952.1	gamma-glutamyltransferase	-
MN092_RS09045 (MN092_09045)	1779191..1779835	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T238030 WP_003180879.1 NZ_CP093290:c1775234-1774488 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T238030 NZ_LR890189:781803-782213 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6945.04 Da
• Isoelectric Point: 10.9186

>AT238030 WP_003180877.1 NZ_CP093290:c1774488-1774315 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT238030 NZ_LR890189:781556-781822 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: T5HT37