Detailed information of TA system TA238024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1374305..1375221
• Replicon: chromosome
• Accession: NZ_CP093289
• Organism: Bacillus subtilis strain H2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: MNG38_RS07285
• Protein ID: WP_015252263.1
• Coordinates: 1374475..1375221 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: MNG38_RS07280
• Protein ID: WP_003232646.1
• Coordinates: 1374305..1374475 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNG38_RS07245 (1371165)	1371165..1371494	+	330	WP_015252268.1	XkdW family protein	-
MNG38_RS07250 (1371491)	1371491..1371655	+	165	WP_015252267.1	XkdX family protein	-
MNG38_RS07255 (1371702)	1371702..1372541	+	840	WP_242139220.1	phage-like element PBSX protein XepA	-
MNG38_RS07260 (1372594)	1372594..1372863	+	270	WP_015252265.1	hemolysin XhlA family protein	-
MNG38_RS07265 (1372876)	1372876..1373139	+	264	WP_003232653.1	phage holin	-
MNG38_RS07270 (1373152)	1373152..1374045	+	894	WP_014479567.1	N-acetylmuramoyl-L-alanine amidase	-
MNG38_RS07275 (1374082)	1374082..1374219	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
MNG38_RS07280 (1374305)	1374305..1374475	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
MNG38_RS07285 (1374475)	1374475..1375221	-	747	WP_015252263.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
MNG38_RS07290 (1375331)	1375331..1376332	-	1002	WP_003232642.1	inorganic phosphate transporter	-
MNG38_RS07295 (1376345)	1376345..1376962	-	618	WP_003218470.1	DUF47 domain-containing protein	-
MNG38_RS07300 (1377238)	1377238..1378554	-	1317	WP_029317680.1	serine/threonine exchanger	-
MNG38_RS07305 (1378943)	1378943..1379893	+	951	WP_014476561.1	ring-cleaving dioxygenase	-
MNG38_RS07310 (1380002)	1380002..1380097	+	96	Protein_1379	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1339012..1395667	56655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29060.56 Da
• Isoelectric Point: 4.7741

>T238024 WP_015252263.1 NZ_CP093289:c1375221-1374475 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNKMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T238024 NZ_LR890185:c4801171-4800887 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT238024 WP_003232646.1 NZ_CP093289:c1374475-1374305 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT238024 NZ_LR890185:c4801403-4801161 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAACAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8