Detailed information of TA system TA023802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TacT2-ataR/DUF1778(antitoxin)
• Location: 1429750..1430543
• Replicon: chromosome
• Accession: NC_012691
• Organism: Tolumonas auensis DSM 9187

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: C4LEA0
• Locus tag: TOLA_RS06660
• Protein ID: WP_012729520.1
• Coordinates: 1429750..1430247 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: C4LEA1
• Locus tag: TOLA_RS06665
• Protein ID: WP_012729521.1
• Coordinates: 1430247..1430543 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOLA_RS06625	1425090..1425410	-	321	WP_041609484.1	hypothetical protein	-
TOLA_RS06630	1425492..1426121	-	630	WP_012729514.1	LysE family transporter	-
TOLA_RS06635	1426245..1426697	-	453	WP_012729515.1	GNAT family N-acetyltransferase	-
TOLA_RS06640	1426764..1427714	-	951	WP_012729516.1	pseudouridine synthase	-
TOLA_RS06645	1427862..1428212	-	351	WP_012729517.1	DUF3147 family protein	-
TOLA_RS06650	1428243..1428665	-	423	WP_012729518.1	VOC family protein	-
TOLA_RS06655	1428716..1429660	-	945	WP_012729519.1	DMT family transporter	-
TOLA_RS06660	1429750..1430247	-	498	WP_012729520.1	GNAT family N-acetyltransferase	Toxin
TOLA_RS06665	1430247..1430543	-	297	WP_012729521.1	DUF1778 domain-containing protein	Antitoxin
TOLA_RS06670	1430654..1431058	-	405	WP_012729522.1	NUDIX domain-containing protein	-
TOLA_RS06675	1431161..1432000	-	840	WP_012729523.1	sulfurtransferase	-
TOLA_RS06680	1432110..1432406	-	297	WP_012729524.1	Dabb family protein	-
TOLA_RS06685	1432435..1433232	-	798	WP_012729525.1	FRG domain-containing protein	-
TOLA_RS06690	1433229..1434056	-	828	WP_012729526.1	hypothetical protein	-
TOLA_RS06695	1434163..1434882	-	720	WP_012729527.1	NYN domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (11-98)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18633.67 Da
• Isoelectric Point: 9.5170

>T23802 WP_012729520.1 NC_012691:c1430247-1429750 [Tolumonas auensis DSM 9187]
MPVSKPEVLHEKHLLEQFDCGNQTLNEWLLRHARQAQSSGSAKTFVVCEDNIVVGYFSLTVGQVETYDAPERIRRGMGQY
PIPVVILARLAVSKMHQGSGIGVGMLQDAIRRTMMISEQVGIRALLTHPIDETASKFYERFGFISSPVRERQLVLLLKDA
RKLIR

Nucleotide

• Length: 498 bp

>T23802 NC_012691:c1430247-1429750 [Tolumonas auensis DSM 9187]
ATGCCTGTTTCAAAACCAGAGGTGTTACATGAGAAACATCTTCTTGAGCAATTTGATTGCGGCAACCAAACATTGAACGA
ATGGCTTTTGCGCCATGCCAGACAAGCACAATCAAGTGGTTCGGCTAAAACTTTTGTCGTTTGTGAAGACAACATTGTTG
TGGGTTATTTCAGTCTCACTGTGGGGCAAGTCGAAACGTATGACGCACCTGAACGCATTCGCCGGGGCATGGGGCAATAT
CCTATTCCTGTCGTTATTCTGGCTCGTTTGGCTGTATCGAAAATGCACCAAGGGTCAGGGATTGGCGTCGGTATGTTGCA
AGATGCCATCCGCCGCACAATGATGATTTCTGAGCAGGTCGGCATCAGAGCGCTGCTTACTCATCCTATTGATGAAACTG
CATCGAAATTTTACGAACGGTTTGGCTTTATTTCATCGCCAGTGAGAGAAAGACAACTGGTGTTATTACTTAAAGACGCA
CGAAAACTCATCCGTTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10863.12 Da
• Isoelectric Point: 5.0621

>AT23802 WP_012729521.1 NC_012691:c1430543-1430247 [Tolumonas auensis DSM 9187]
MASSASSVSRTSRSARLGLRATPEQEAVLRRAADISHKSLTDFILDSACHAAEQTLLDQRLFVVSGEQYQTLLDMLDQPA
QENQGLQDLFSRTAPWEK

Nucleotide

• Length: 297 bp

>AT23802 NC_012691:c1430543-1430247 [Tolumonas auensis DSM 9187]
ATGGCCAGTTCTGCATCTTCGGTTTCACGAACTTCACGGTCTGCTCGTCTCGGGCTTCGGGCTACACCTGAGCAAGAGGC
AGTACTACGTCGCGCTGCTGACATTTCGCACAAATCATTAACTGATTTCATTCTTGATAGTGCATGCCATGCGGCTGAAC
AAACCCTCCTTGATCAGCGTTTATTCGTTGTTTCCGGTGAACAATATCAAACCCTGCTTGATATGCTTGATCAACCCGCT
CAAGAAAATCAGGGCTTGCAAGACCTTTTCTCCCGCACTGCACCTTGGGAGAAATAA

Structures

Toxin

• AlphaFold DB: C4LEA0

Antitoxin

• AlphaFold DB: C4LEA1