238018

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 84084..85707 Replicon plasmid pEC-14-2-9-2
Accession NZ_CP093284
Organism Escherichia coli strain EC-14-2-9

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag MNY25_RS24095 Protein ID WP_000088046.1
Coordinates 84382..85707 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag MNY25_RS24090 Protein ID WP_024136327.1
Coordinates 84084..84377 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY25_RS24065 (MNY25_24065) 79956..81197 + 1242 WP_001053338.1 VWA domain-containing protein -
MNY25_RS26040 81288..81419 + 132 WP_000374059.1 hypothetical protein -
MNY25_RS24070 (MNY25_24070) 81760..82041 - 282 WP_000077926.1 hypothetical protein -
MNY25_RS24075 (MNY25_24075) 82091..82282 - 192 WP_000398480.1 hypothetical protein -
MNY25_RS24080 (MNY25_24080) 82374..82715 - 342 WP_001151575.1 hypothetical protein -
MNY25_RS24085 (MNY25_24085) 83088..83480 + 393 WP_000341066.1 hypothetical protein -
MNY25_RS24090 (MNY25_24090) 84084..84377 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
MNY25_RS24095 (MNY25_24095) 84382..85707 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
MNY25_RS24100 (MNY25_24100) 85768..85974 + 207 WP_000134171.1 hypothetical protein -
MNY25_RS24105 (MNY25_24105) 86076..86486 + 411 WP_000985911.1 hypothetical protein -
MNY25_RS24110 (MNY25_24110) 86499..87314 + 816 WP_053276223.1 HNH endonuclease signature motif containing protein -
MNY25_RS24115 (MNY25_24115) 87598..88295 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
MNY25_RS24120 (MNY25_24120) 88339..89004 - 666 WP_000490638.1 hypothetical protein -
MNY25_RS24125 (MNY25_24125) 89062..89442 - 381 WP_001426317.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA17 / aadA5 / sul2 / floR / aadA2 / qacE / sul1 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-64 - 1..248308 248308
- flank IS/Tn - - 87792..88295 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T238018 WP_000088046.1 NZ_CP093284:84382-85707 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T238018 NZ_LR890185:776165-776575 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT238018 WP_024136327.1 NZ_CP093284:84084-84377 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT238018 NZ_LR890185:775918-776184 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References