Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4384595..4385092 Replicon chromosome
Accession NZ_CP093283
Organism Escherichia coli strain EC-14-2-9

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MNY25_RS21245 Protein ID WP_001173343.1
Coordinates 4384595..4384768 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MNY25_RS21250 Protein ID WP_000398619.1
Coordinates 4384796..4385092 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY25_RS21235 (4381265) 4381265..4382722 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
MNY25_RS21240 (4382959) 4382959..4384476 + 1518 WP_001295074.1 lysine--tRNA ligase -
MNY25_RS21245 (4384595) 4384595..4384768 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MNY25_RS21250 (4384796) 4384796..4385092 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MNY25_RS21255 (4385319) 4385319..4385591 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
MNY25_RS21260 (4385603) 4385603..4385833 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MNY25_RS21265 (4386014) 4386014..4387645 + 1632 WP_001216477.1 sensor histidine kinase -
MNY25_RS21270 (4387642) 4387642..4388361 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T238017 WP_001173343.1 NZ_CP093283:c4384768-4384595 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T238017 NZ_LR890185:710080-710565 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTAATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT238017 WP_000398619.1 NZ_CP093283:c4385092-4384796 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT238017 NZ_LR890185:709790-710083 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References