Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4166989..4167565 Replicon chromosome
Accession NZ_CP093283
Organism Escherichia coli strain EC-14-2-9

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag MNY25_RS20110 Protein ID WP_001297643.1
Coordinates 4167278..4167565 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag MNY25_RS20105 Protein ID WP_000063160.1
Coordinates 4166989..4167291 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY25_RS20090 (4163585) 4163585..4165735 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
MNY25_RS20095 (4165785) 4165785..4165988 + 204 WP_000467859.1 YbdD/YjiX family protein -
MNY25_RS20100 (4165999) 4165999..4166955 + 957 WP_001297640.1 GTPase -
MNY25_RS20105 (4166989) 4166989..4167291 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
MNY25_RS20110 (4167278) 4167278..4167565 - 288 WP_001297643.1 BrnT family toxin Toxin
MNY25_RS26010 (4167764) 4167764..4168460 - 697 Protein_3958 IS1 family transposase -
MNY25_RS20125 (4168775) 4168775..4170007 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
MNY25_RS20130 (4170048) 4170048..4171328 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4167764..4167973 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T238015 WP_001297643.1 NZ_CP093283:c4167565-4167278 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T238015 NZ_LR890184:c4598908-4598624 [Klebsiella aerogenes]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCTGCAGATTGTGCAGAATCCGCGAATGGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT238015 WP_000063160.1 NZ_CP093283:c4167291-4166989 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT238015 NZ_LR890184:c4599140-4598898 [Klebsiella aerogenes]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTGTTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References