Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3536402..3537020 Replicon chromosome
Accession NZ_CP093283
Organism Escherichia coli strain EC-14-2-9

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MNY25_RS17070 Protein ID WP_001291435.1
Coordinates 3536802..3537020 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MNY25_RS17065 Protein ID WP_000344800.1
Coordinates 3536402..3536776 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY25_RS17055 (3531491) 3531491..3532684 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MNY25_RS17060 (3532707) 3532707..3535856 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MNY25_RS17065 (3536402) 3536402..3536776 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MNY25_RS17070 (3536802) 3536802..3537020 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MNY25_RS17075 (3537192) 3537192..3537743 + 552 WP_000102564.1 maltose O-acetyltransferase -
MNY25_RS17080 (3537859) 3537859..3538329 + 471 WP_000136192.1 YlaC family protein -
MNY25_RS17085 (3538493) 3538493..3540043 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MNY25_RS17090 (3540085) 3540085..3540438 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MNY25_RS17100 (3540817) 3540817..3541128 + 312 WP_000409911.1 MGMT family protein -
MNY25_RS17105 (3541159) 3541159..3541731 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T238012 WP_001291435.1 NZ_CP093283:3536802-3537020 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T238012 NZ_LR890184:1844211-1844314 [Klebsiella aerogenes]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT238012 WP_000344800.1 NZ_CP093283:3536402-3536776 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT238012 NZ_LR890184:c1844322-1844176 [Klebsiella aerogenes]
TAATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAA
GTTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTGACTGTGACAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References