Detailed information of TA system TA238011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2506526..2507164
• Replicon: chromosome
• Accession: NZ_CP093283
• Organism: Escherichia coli strain EC-14-2-9

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: MNY25_RS12070
• Protein ID: WP_001447010.1
• Coordinates: 2506988..2507164 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MNY25_RS12065
• Protein ID: WP_001270286.1
• Coordinates: 2506526..2506942 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY25_RS12045 (2501678)	2501678..2502619	-	942	WP_001251304.1	ABC transporter permease	-
MNY25_RS12050 (2502620)	2502620..2503633	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
MNY25_RS12055 (2503651)	2503651..2504796	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
MNY25_RS12060 (2505041)	2505041..2506447	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
MNY25_RS12065 (2506526)	2506526..2506942	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MNY25_RS12070 (2506988)	2506988..2507164	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MNY25_RS12075 (2507386)	2507386..2507616	+	231	WP_000494244.1	YncJ family protein	-
MNY25_RS12080 (2507708)	2507708..2509669	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MNY25_RS12085 (2509742)	2509742..2510278	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
MNY25_RS12090 (2510370)	2510370..2511545	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T238011 WP_001447010.1 NZ_CP093283:c2507164-2506988 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T238011 NZ_LR890184:835334-835744 [Klebsiella aerogenes]
GTGGTCCTGTGGCAATCTGATTTACGTATTTCCTGGCGTTCGCAGTGGTTCTCGCTGCTGCTGCACGGGGTGGTGGCGGC
GATAGTGCTGCTAATGCCCTGGCCGTTAAGCTACACCCCGATTTGGCTCCTGTTGCTATCGCTGGTGGTGTTTGACTGCG
TGCGCAGCCAGCGGCGGATCCACGCCTGCCAGGGAGAAATTAAGCTGTTGATCGATTCCCGTTTACGGTGGCAAAAAACG
GAATGGGATATCGTCGGGACGCCATGGGTTATCAGTAGCGGTATGCTGCTGCGCCTGAAGCATGCGGAAACCGGCCGTAG
CCAGCATTTGTGGGTGGCGGCGGATAGCATGGATGCGGGGGAATGGCGGGATTTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCACGACTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT238011 WP_001270286.1 NZ_CP093283:c2506942-2506526 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT238011 NZ_LR890184:835087-835353 [Klebsiella aerogenes]
ATGGACATTAACAACAAAGCTCGCATCCACTGGGCATGCCGCCGCGGTATGCGCGAACTCGATATCTCCATCATGCCGTT
TTTCGAGTATGAGTACGACACGCTCAGCGATGACGATAAGCGTCTGTTCATCCGTTTGCTGGAGAACGATGATCCGGATC
TGTTTAACTGGCTGATGAATCACGGTAAGCCGGCAGATGCGCAACTACAACGGATGATAAATTTAATCCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8