Detailed information of TA system TA023801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 784610..785373
• Replicon: chromosome
• Accession: NC_012691
• Organism: Tolumonas auensis DSM 9187

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: TOLA_RS03605
• Protein ID: WP_148210409.1
• Coordinates: 784876..785373 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: C4LB94
• Locus tag: TOLA_RS03600
• Protein ID: WP_012728928.1
• Coordinates: 784610..784879 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOLA_RS03555	779826..780101	-	276	WP_012728919.1	DUF1294 domain-containing protein	-
TOLA_RS03560	780153..780548	-	396	WP_012728920.1	GFA family protein	-
TOLA_RS03565	780646..780930	-	285	WP_012728921.1	hypothetical protein	-
TOLA_RS03570	780984..781409	-	426	WP_012728922.1	aldehyde-activating protein	-
TOLA_RS03575	781427..781729	-	303	WP_012728923.1	hypothetical protein	-
TOLA_RS03580	781740..782213	-	474	WP_012728924.1	DUF1810 domain-containing protein	-
TOLA_RS03585	782309..783019	-	711	WP_012728925.1	hypothetical protein	-
TOLA_RS03590	783012..783572	-	561	WP_012728926.1	cyclin-dependent kinase inhibitor 3 family protein	-
TOLA_RS03595	783679..784425	-	747	WP_012728927.1	class I SAM-dependent methyltransferase	-
TOLA_RS03600	784610..784879	+	270	WP_012728928.1	DUF1778 domain-containing protein	Antitoxin
TOLA_RS03605	784876..785373	+	498	WP_148210409.1	GNAT family N-acetyltransferase	Toxin
TOLA_RS03610	785456..787222	-	1767	WP_012728930.1	ATP-binding protein	-
TOLA_RS03615	787328..788116	-	789	WP_083757722.1	IS3 family transposase	-
TOLA_RS03620	788151..788495	-	345	WP_012728932.1	transposase	-
TOLA_RS03625	788570..789601	-	1032	WP_012728933.1	reverse transcriptase family protein	-
TOLA_RS03630	789830..790180	-	351	WP_012728934.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (37-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-86)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18548.19 Da
• Isoelectric Point: 6.9791

>T23801 WP_148210409.1 NC_012691:784876-785373 [Tolumonas auensis DSM 9187]
IMMTVKLDKATHDRQHFDCGVESLNNYLKVMANQQSQKDNTRTFVLVEEQNSERVIGYYTLTMTPIDLSALPDKLQKKHH
NASSGGLIARLAIDKRYAKQGHGEWLLIDALSRLLQASETVAFPVVIVDAKDGAIGFYEKFGFTPFQDSTNKLFLTIADI
RASLE

Nucleotide

• Length: 498 bp

>T23801 NC_012691:784876-785373 [Tolumonas auensis DSM 9187]
ATAATGATGACGGTGAAGCTGGATAAAGCAACGCATGACCGACAGCATTTTGATTGTGGTGTAGAAAGCCTGAATAACTA
TTTGAAAGTGATGGCCAACCAGCAATCACAGAAAGATAACACGCGAACATTTGTGCTCGTAGAAGAGCAAAATTCAGAAC
GGGTGATTGGTTATTACACGTTAACCATGACACCAATAGACCTCAGTGCATTACCCGATAAATTGCAAAAAAAGCATCAC
AATGCCAGTTCAGGCGGACTGATTGCTCGTTTAGCTATAGATAAACGCTATGCAAAACAAGGTCACGGAGAGTGGTTGCT
GATTGATGCTTTGAGCCGATTACTGCAAGCCAGTGAAACCGTGGCTTTTCCGGTCGTTATTGTTGATGCGAAAGATGGTG
CAATTGGCTTTTATGAAAAGTTTGGATTTACGCCATTTCAAGATTCAACTAATAAGCTGTTTCTTACAATTGCTGATATT
CGCGCCAGTCTTGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9656.20 Da
• Isoelectric Point: 10.2690

>AT23801 WP_012728928.1 NC_012691:784610-784879 [Tolumonas auensis DSM 9187]
MATALPRITARVDAETQDLLAQAAAIAGMTSINSFVLNAAIDKAKQIMERERALKLSQRDAMMLADALDAPTKPNARLQQ
AAERYKAKT

Nucleotide

• Length: 270 bp

>AT23801 NC_012691:784610-784879 [Tolumonas auensis DSM 9187]
ATGGCAACCGCATTACCTCGTATCACTGCGCGTGTTGACGCAGAAACACAAGATTTACTTGCTCAAGCGGCAGCGATTGC
TGGTATGACGAGTATCAACTCATTTGTACTTAATGCAGCCATCGATAAAGCAAAACAAATCATGGAACGTGAAAGAGCGC
TAAAACTGTCTCAACGTGATGCCATGATGTTAGCTGATGCGCTGGACGCACCAACAAAACCGAATGCCAGATTGCAACAG
GCCGCAGAACGCTACAAGGCTAAAACATAA

Structures

Antitoxin

• AlphaFold DB: C4LB94