Detailed information of TA system TA023800

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE(toxin)
• Location: 612468..612990
• Replicon: chromosome
• Accession: NC_012691
• Organism: Tolumonas auensis DSM 9187

Toxin (Protein)

• Gene name: relE
• Uniprot ID: C4LA44
• Locus tag: TOLA_RS02810
• Protein ID: WP_012728772.1
• Coordinates: 612709..612990 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C4LA43
• Locus tag: TOLA_RS02805
• Protein ID: WP_012728771.1
• Coordinates: 612468..612719 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TOLA_RS16765	608369..608467	-	99	Protein_535	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
TOLA_RS02790	608764..610050	-	1287	WP_012728768.1	phosphoribosylamine--glycine ligase	-
TOLA_RS02795	610414..612006	-	1593	WP_012728769.1	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	-
TOLA_RS02805	612468..612719	+	252	WP_012728771.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
TOLA_RS02810	612709..612990	+	282	WP_012728772.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TOLA_RS02815	613283..613756	-	474	WP_012728773.1	Lrp/AsnC family transcriptional regulator	-
TOLA_RS02820	614066..615733	-	1668	WP_012728775.1	energy-dependent translational throttle protein EttA	-
TOLA_RS02830	616134..616655	+	522	WP_012728776.1	RDD family protein	-
TOLA_RS02835	616698..617768	-	1071	WP_012728777.1	LPS export ABC transporter permease LptG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10924.63 Da
• Isoelectric Point: 10.7594

>T23800 WP_012728772.1 NC_012691:612709-612990 [Tolumonas auensis DSM 9187]
MTYRLKFEKRAWKEWEKLGHPVRDQFKKKLAERLKNPRVPSAKLSGHADRYKIKLRSAGYRLVYQVFDDEVVVSVIAVGK
RENSDIYNTASGR

Nucleotide

• Length: 282 bp

>T23800 NC_012691:612709-612990 [Tolumonas auensis DSM 9187]
ATGACCTATAGACTGAAATTTGAGAAAAGAGCCTGGAAAGAGTGGGAGAAGCTAGGTCATCCGGTCAGAGATCAGTTTAA
AAAGAAGCTGGCCGAGCGCCTGAAAAATCCTCGTGTTCCGAGTGCCAAGCTCAGTGGTCATGCTGATCGTTATAAAATCA
AGTTGCGGTCAGCTGGCTATCGGTTGGTTTATCAGGTGTTCGATGATGAAGTGGTTGTCAGTGTGATCGCCGTTGGTAAG
CGCGAAAATTCGGATATTTATAATACGGCCAGCGGGCGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9247.49 Da
• Isoelectric Point: 3.8345

>AT23800 WP_012728771.1 NC_012691:612468-612719 [Tolumonas auensis DSM 9187]
MAYQILTSVAASITELKKNPMGTAMEGEGQPVAILNRNEPAFYIVPPALFGYFQELAEDAELNRIADERMNNPEFIEISI
DDL

Nucleotide

• Length: 252 bp

>AT23800 NC_012691:612468-612719 [Tolumonas auensis DSM 9187]
ATGGCATATCAAATTCTGACATCTGTTGCAGCCAGCATCACTGAGCTGAAGAAAAATCCGATGGGAACGGCAATGGAGGG
GGAAGGTCAGCCGGTCGCTATTCTGAATCGCAATGAACCGGCTTTTTATATCGTCCCGCCTGCATTGTTTGGCTATTTTC
AGGAGTTAGCTGAGGATGCTGAATTGAATCGGATCGCTGATGAACGAATGAATAATCCTGAGTTTATCGAGATCAGTATT
GATGACCTATAG

Structures

Toxin

• AlphaFold DB: C4LA44

Antitoxin

• AlphaFold DB: C4LA43