Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 999561..1000215 Replicon chromosome
Accession NZ_CP093283
Organism Escherichia coli strain EC-14-2-9

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MNY25_RS04955 Protein ID WP_000244777.1
Coordinates 999808..1000215 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MNY25_RS04950 Protein ID WP_000354046.1
Coordinates 999561..999827 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY25_RS04925 (994730) 994730..995473 + 744 WP_000951964.1 SDR family oxidoreductase -
MNY25_RS04930 (995530) 995530..996963 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
MNY25_RS04935 (997008) 997008..997319 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MNY25_RS04940 (997483) 997483..998142 + 660 WP_000250274.1 hemolysin III family protein -
MNY25_RS04945 (998338) 998338..999318 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MNY25_RS04950 (999561) 999561..999827 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MNY25_RS04955 (999808) 999808..1000215 + 408 WP_000244777.1 protein YgfX Toxin
MNY25_RS04960 (1000255) 1000255..1000776 - 522 WP_001055874.1 flavodoxin FldB -
MNY25_RS04965 (1000888) 1000888..1001784 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MNY25_RS04970 (1001809) 1001809..1002519 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MNY25_RS04975 (1002525) 1002525..1004258 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T237998 WP_000244777.1 NZ_CP093283:999808-1000215 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T237998 NZ_LR883966:c75065-74685 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT237998 WP_000354046.1 NZ_CP093283:999561-999827 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT237998 NZ_LR883966:c75286-75065 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References