Detailed information of TA system TA237990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 5949323..5950362
• Replicon: chromosome
• Accession: NZ_CP093280
• Organism: Pseudomonas sp. T1-3-2

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: LZ023_RS27115
• Protein ID: WP_268143435.1
• Coordinates: 5949323..5949898 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: LZ023_RS27120
• Protein ID: WP_268143437.1
• Coordinates: 5949895..5950362 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZ023_RS27090 (LZ023_27050)	5945763..5946488	-	726	WP_268143429.1	CBASS effector endonuclease NucC	-
LZ023_RS27095 (LZ023_27055)	5946525..5947427	-	903	WP_268143430.1	CBASS oligonucleotide cyclase	-
LZ023_RS27100 (LZ023_27060)	5947427..5947927	-	501	WP_268143431.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
LZ023_RS27105 (LZ023_27065)	5947924..5948394	-	471	WP_268151637.1	hypothetical protein	-
LZ023_RS27110 (LZ023_27070)	5948394..5949305	-	912	WP_268143433.1	AAA family ATPase	-
LZ023_RS27115 (LZ023_27075)	5949323..5949898	-	576	WP_268143435.1	PIN domain-containing protein	Toxin
LZ023_RS27120 (LZ023_27080)	5949895..5950362	-	468	WP_268143437.1	helix-turn-helix domain-containing protein	Antitoxin
LZ023_RS27125 (LZ023_27085)	5950576..5950959	+	384	WP_268143439.1	RAQPRD family integrative conjugative element protein	-
LZ023_RS27130 (LZ023_27090)	5950956..5951189	+	234	WP_268143440.1	TIGR03758 family integrating conjugative element protein	-
LZ023_RS27135 (LZ023_27095)	5951206..5951565	+	360	WP_268143441.1	TIGR03745 family integrating conjugative element membrane protein	-
LZ023_RS27140 (LZ023_27100)	5951578..5951976	+	399	WP_268143443.1	TIGR03750 family conjugal transfer protein	-
LZ023_RS27145 (LZ023_27105)	5951973..5952662	+	690	WP_268143444.1	TIGR03746 family integrating conjugative element protein	-
LZ023_RS27150 (LZ023_27110)	5952659..5953585	+	927	WP_268143445.1	TIGR03749 family integrating conjugative element protein	-
LZ023_RS27155 (LZ023_27115)	5953575..5954996	+	1422	WP_268143446.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5909932..6030712	120780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• SinI-like, DNA-binding domain (84-131)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21600.61 Da
• Isoelectric Point: 5.6513

>T237990 WP_268143435.1 NZ_CP093280:c5949898-5949323 [Pseudomonas sp. T1-3-2]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQTIHEEWKRNLLINRPDLTRAQVDRTSDLMDRAIPDGLVEGY
ESLAAGLTLPDPDDRHVLAAAIRCGASVIVTFNQRDFPNELLAPYGVESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLRN
PTIDVDRYLDILLRQGLVQTTKVLASYRTIL

Nucleotide

• Length: 576 bp

>T237990 NZ_LR883965:3181413-3181520 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17277.66 Da
• Isoelectric Point: 5.6017

>AT237990 WP_268143437.1 NZ_CP093280:c5950362-5949895 [Pseudomonas sp. T1-3-2]
MTVTAQSKMTLPAEGEVKAAVQGQRALAAYLATQFETQHIQIFDDQKQAHQVELPTSALRLLVDILAELADGNAVKVVPV
YAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELTQQSQELGMGYE

Nucleotide

• Length: 468 bp

>AT237990 NZ_LR883965:c3181356-3181299 [Escherichia coli]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT