Detailed information of TA system TA023799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4500005..4500675
• Replicon: chromosome
• Accession: NC_012669
• Organism: Beutenbergia cavernae DSM 12333

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5C5V2
• Locus tag: BCAV_RS20485
• Protein ID: WP_015884547.1
• Coordinates: 4500244..4500675 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C5C5V1
• Locus tag: BCAV_RS20480
• Protein ID: WP_015884546.1
• Coordinates: 4500005..4500247 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BCAV_RS20455	4495411..4496370	-	960	WP_015884541.1	class I SAM-dependent methyltransferase	-
BCAV_RS20460	4496516..4497088	-	573	WP_015884542.1	TetR/AcrR family transcriptional regulator	-
BCAV_RS20465	4497186..4498058	+	873	WP_015884543.1	LLM class F420-dependent oxidoreductase	-
BCAV_RS20470	4498138..4499295	-	1158	WP_015884544.1	serine hydrolase	-
BCAV_RS20475	4499316..4499798	-	483	WP_015884545.1	DUF1269 domain-containing protein	-
BCAV_RS20480	4500005..4500247	+	243	WP_015884546.1	hypothetical protein	Antitoxin
BCAV_RS20485	4500244..4500675	+	432	WP_015884547.1	PIN domain-containing protein	Toxin
BCAV_RS20490	4500762..4502123	+	1362	WP_015884548.1	fuconate dehydratase	-
BCAV_RS20495	4502120..4502890	+	771	WP_015884549.1	SDR family oxidoreductase	-
BCAV_RS20500	4502901..4503905	+	1005	WP_015884550.1	aldo/keto reductase	-
BCAV_RS20505	4503920..4504855	-	936	WP_015884551.1	aldo/keto reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15249.32 Da
• Isoelectric Point: 6.2090

>T23799 WP_015884547.1 NC_012669:4500244-4500675 [Beutenbergia cavernae DSM 12333]
VIVDANVLLYAVDTGSPFHRAAKEWLEEALSGPARVGLPWPSLLAFQRIASHPRAAAQPLTPGAAWAFITEWLRADPAWI
PTPGDGHAAILGRLLTAGDLRGNLVTDAHLAALALEHGVGICSFDSDFARFDGLTWHNPSAAR

Nucleotide

• Length: 432 bp

>T23799 NC_012669:4500244-4500675 [Beutenbergia cavernae DSM 12333]
GTGATCGTCGACGCGAACGTGCTGCTGTACGCCGTCGACACCGGCAGCCCGTTCCACCGCGCCGCCAAGGAATGGCTGGA
GGAGGCACTGAGCGGACCGGCCCGGGTCGGCCTTCCGTGGCCCTCGCTCCTCGCGTTCCAACGGATCGCCTCCCACCCGC
GAGCCGCAGCACAGCCGCTGACCCCAGGGGCCGCGTGGGCGTTCATCACCGAGTGGCTGCGCGCGGATCCAGCCTGGATC
CCGACGCCGGGTGACGGGCATGCGGCGATCCTTGGCCGACTCCTCACCGCCGGGGACCTCCGCGGCAACCTGGTCACGGA
CGCCCACCTCGCCGCGCTCGCGCTCGAGCACGGCGTCGGCATCTGCTCGTTCGACAGTGACTTCGCCCGCTTCGACGGGC
TGACCTGGCACAACCCGAGCGCAGCGCGTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8929.01 Da
• Isoelectric Point: 5.7300

>AT23799 WP_015884546.1 NC_012669:4500005-4500247 [Beutenbergia cavernae DSM 12333]
MRTTIRLDDDVAAAAERLRRERHIGLGEAVNELARAGLHRGAPAERRYRQRTADLGLRIDVSNVAEALELLDEADLDAPR

Nucleotide

• Length: 243 bp

>AT23799 NC_012669:4500005-4500247 [Beutenbergia cavernae DSM 12333]
ATGCGCACGACGATCAGGCTCGACGACGACGTCGCGGCCGCCGCGGAGCGGCTTCGGCGCGAGCGTCACATCGGCCTGGG
CGAAGCCGTCAACGAGCTGGCGCGGGCGGGCTTGCATCGCGGCGCACCTGCGGAGCGCCGATACCGGCAGCGGACGGCGG
ACCTCGGTCTGCGGATCGACGTCTCCAACGTGGCCGAGGCTCTCGAGCTCCTCGATGAGGCCGACCTGGACGCGCCACGG
TGA

Structures

Toxin

• AlphaFold DB: C5C5V2

Antitoxin

• AlphaFold DB: C5C5V1