Detailed information of TA system TA237984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 44128..44771
• Replicon: plasmid p2
• Accession: NZ_CP093278
• Organism: Raoultella sp. HC6

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: MMW58_RS29810
• Protein ID: WP_094309882.1
• Coordinates: 44355..44771 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: MMW58_RS29805
• Protein ID: WP_049010641.1
• Coordinates: 44128..44358 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMW58_RS29770	39139..39894	-	756	WP_001568031.1	replication initiation protein RepE	-
MMW58_RS29775	40634..40747	+	114	WP_162180131.1	hypothetical protein	-
MMW58_RS29780	40876..41133	+	258	WP_032700766.1	hypothetical protein	-
MMW58_RS29785	41190..41978	-	789	WP_047722707.1	site-specific integrase	-
MMW58_RS29790	41975..42304	-	330	WP_032700767.1	hypothetical protein	-
MMW58_RS29795	42533..43141	-	609	WP_032700768.1	hypothetical protein	-
MMW58_RS29800	43204..43557	-	354	WP_032700769.1	hypothetical protein	-
MMW58_RS29805	44128..44358	+	231	WP_049010641.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MMW58_RS29810	44355..44771	+	417	WP_094309882.1	type II toxin-antitoxin system VapC family toxin	Toxin
MMW58_RS29815	44803..45159	+	357	WP_179222107.1	hypothetical protein	-
MMW58_RS29820	45639..45891	+	253	Protein_56	ISNCY family transposase	-
MMW58_RS29825	46015..46476	-	462	WP_094309881.1	Mov34/MPN/PAD-1 family protein	-
MMW58_RS29830	46463..48247	-	1785	WP_200557577.1	E2/UBC family protein	-
MMW58_RS29835	48252..49544	-	1293	WP_200557576.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..100650	100650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-128)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.53 Da
• Isoelectric Point: 7.8874

>T237984 WP_094309882.1 NZ_CP093278:44355-44771 [Raoultella sp. HC6]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARVDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVLGLNLEDWVK

Nucleotide

• Length: 417 bp

>T237984 NZ_LR883965:1948651-1948929 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8681.76 Da
• Isoelectric Point: 4.4692

>AT237984 WP_049010641.1 NZ_CP093278:44128-44358 [Raoultella sp. HC6]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFASLEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT237984 NZ_LR883965:1948388-1948648 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA