Detailed information of TA system TA237983

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 158295..159031
• Replicon: plasmid p1
• Accession: NZ_CP093277
• Organism: Raoultella sp. HC6

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: MMW58_RS29500
• Protein ID: WP_004098919.1
• Coordinates: 158549..159031 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: MMW58_RS29495
• Protein ID: WP_241723802.1
• Coordinates: 158295..158561 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMW58_RS29450	153475..154401	+	927	WP_224373622.1	Abi family protein	-
MMW58_RS29455	154615..155199	-	585	WP_223862240.1	hypothetical protein	-
MMW58_RS29460	155483..155746	+	264	WP_009310051.1	hypothetical protein	-
MMW58_RS29465	155743..156309	+	567	WP_009654312.1	hypothetical protein	-
MMW58_RS29470	156340..156834	+	495	WP_013023776.1	hypothetical protein	-
MMW58_RS29475	156878..157246	+	369	WP_009653915.1	hypothetical protein	-
MMW58_RS29480	157280..157483	+	204	WP_004098931.1	HHA domain-containing protein	-
MMW58_RS29485	157532..157789	+	258	WP_009653918.1	hypothetical protein	-
MMW58_RS29490	157865..158119	+	255	WP_009653916.1	hypothetical protein	-
MMW58_RS29495	158295..158561	+	267	WP_241723802.1	DUF1778 domain-containing protein	Antitoxin
MMW58_RS29500	158549..159031	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
MMW58_RS29505	159190..160722	-	1533	WP_241723803.1	IS3 family transposase	-
MMW58_RS29510	161241..162239	-	999	WP_020325103.1	LacI family DNA-binding transcriptional regulator	-
MMW58_RS29515	162435..162908	-	474	WP_241723804.1	substrate-binding domain-containing protein	-
MMW58_RS29520	163281..163978	+	698	Protein_177	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..167204	167204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T237983 WP_004098919.1 NZ_CP093277:158549-159031 [Raoultella sp. HC6]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T237983 NZ_LR883965:1728695-1729237 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10119.52 Da
• Isoelectric Point: 4.5141

>AT237983 WP_241723802.1 NZ_CP093277:158295-158561 [Raoultella sp. HC6]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPIEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT237983 NZ_LR883965:1728400-1728711 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAAATCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928