Detailed information of TA system TA237982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 5248572..5249148
• Replicon: chromosome
• Accession: NZ_CP093276
• Organism: Raoultella sp. HC6

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: MMW58_RS25400
• Protein ID: WP_241722355.1
• Coordinates: 5248861..5249148 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: MMW58_RS25395
• Protein ID: WP_241722354.1
• Coordinates: 5248572..5248874 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMW58_RS25385	5245077..5247149	+	2073	WP_241722352.1	AAA family ATPase	-
MMW58_RS25390	5247149..5248471	+	1323	WP_241722353.1	restriction endonuclease	-
MMW58_RS25395	5248572..5248874	-	303	WP_241722354.1	BrnA antitoxin family protein	Antitoxin
MMW58_RS25400	5248861..5249148	-	288	WP_241722355.1	BrnT family toxin	Toxin
MMW58_RS25405	5249266..5249685	-	420	WP_133600779.1	FosA family fosfomycin resistance glutathione transferase	-
MMW58_RS25410	5249689..5250588	-	900	WP_133600780.1	LysR family transcriptional regulator	-
MMW58_RS25415	5250676..5251458	+	783	WP_241722356.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (40-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11067.49 Da
• Isoelectric Point: 7.5254

>T237982 WP_241722355.1 NZ_CP093276:c5249148-5248861 [Raoultella sp. HC6]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEHRWQTIGLAHGIIVILVVHSVRFESGAEVIRIIS
ARKADGKERSRYEHS

Nucleotide

• Length: 288 bp

>T237982 NZ_LR883965:1694711-1694929 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11324.78 Da
• Isoelectric Point: 9.7777

>AT237982 WP_241722354.1 NZ_CP093276:c5248874-5248572 [Raoultella sp. HC6]
MSTVKYKRGNPAGLSAQREDELRALANKADGEIDYSDIPASGDERWPDAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMNRDLGEK

Nucleotide

• Length: 303 bp

>AT237982 NZ_LR883965:1694311-1694685 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG