Detailed information of TA system TA237966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4477349..4477968
• Replicon: chromosome
• Accession: NZ_CP093271
• Organism: Klebsiella oxytoca strain C-21-18

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: MN553_RS21245
• Protein ID: WP_004099646.1
• Coordinates: 4477750..4477968 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: MN553_RS21240
• Protein ID: WP_004099648.1
• Coordinates: 4477349..4477723 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MN553_RS21230 (MN553_21230)	4472504..4473697	+	1194	WP_004099653.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
MN553_RS21235 (MN553_21235)	4473720..4476866	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit AcrB	-
MN553_RS21240 (MN553_21240)	4477349..4477723	+	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
MN553_RS21245 (MN553_21245)	4477750..4477968	+	219	WP_004099646.1	HHA domain-containing protein	Toxin
MN553_RS21250 (MN553_21250)	4478129..4478695	+	567	WP_004099644.1	maltose O-acetyltransferase	-
MN553_RS21255 (MN553_21255)	4478664..4478801	-	138	WP_224226720.1	hypothetical protein	-
MN553_RS21260 (MN553_21260)	4478832..4479302	+	471	WP_004099643.1	YlaC family protein	-
MN553_RS21265 (MN553_21265)	4479277..4480731	-	1455	WP_017146481.1	PLP-dependent aminotransferase family protein	-
MN553_RS21270 (MN553_21270)	4480834..4481532	+	699	WP_004099639.1	GNAT family protein	-
MN553_RS21275 (MN553_21275)	4481529..4481669	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
MN553_RS21280 (MN553_21280)	4481669..4481932	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T237966 WP_004099646.1 NZ_CP093271:4477750-4477968 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T237966 NZ_LR883050:3563659-3563877 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT237966 WP_004099648.1 NZ_CP093271:4477349-4477723 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT237966 NZ_LR883050:3563259-3563633 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAATCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0H3H713