Detailed information of TA system TA237955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 90924..91501
• Replicon: plasmid pW17-3-a
• Accession: NZ_CP093261
• Organism: Moellerella wisconsensis strain W17-3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N651
• Locus tag: MNY68_RS16250
• Protein ID: WP_023159957.1
• Coordinates: 91169..91501 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U5N340
• Locus tag: MNY68_RS16245
• Protein ID: WP_023159984.1
• Coordinates: 90924..91169 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY68_RS16205 (MNY68_16205)	85937..86281	+	345	WP_023159966.1	single-stranded DNA-binding protein	-
MNY68_RS16210 (MNY68_16210)	86274..86696	+	423	WP_048821775.1	hypothetical protein	-
MNY68_RS16215 (MNY68_16215)	86706..87098	+	393	WP_048821774.1	hypothetical protein	-
MNY68_RS16220 (MNY68_16220)	87379..87858	+	480	WP_048821772.1	Hcp family type VI secretion system effector	-
MNY68_RS16225 (MNY68_16225)	87867..88445	+	579	WP_048822182.1	hypothetical protein	-
MNY68_RS16230 (MNY68_16230)	89206..89574	+	369	WP_048821769.1	hypothetical protein	-
MNY68_RS16235 (MNY68_16235)	89672..89899	-	228	WP_048821767.1	plasmid partition protein ParG	-
MNY68_RS16240 (MNY68_16240)	90008..90631	-	624	WP_023159958.1	AAA family ATPase	-
MNY68_RS16245 (MNY68_16245)	90924..91169	+	246	WP_023159984.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
MNY68_RS16250 (MNY68_16250)	91169..91501	+	333	WP_023159957.1	endoribonuclease MazF	Toxin
MNY68_RS16255 (MNY68_16255)	91532..91912	+	381	WP_048821763.1	hypothetical protein	-
MNY68_RS16260 (MNY68_16260)	92001..92141	-	141	WP_072022937.1	Hok/Gef family protein	-
MNY68_RS16265 (MNY68_16265)	92461..92919	-	459	WP_048821762.1	hypothetical protein	-
MNY68_RS16270 (MNY68_16270)	93018..93272	-	255	WP_048821760.1	hypothetical protein	-
MNY68_RS16275 (MNY68_16275)	93311..93580	-	270	WP_048821758.1	hypothetical protein	-
MNY68_RS16280 (MNY68_16280)	93874..94827	+	954	WP_048821757.1	site-specific integrase	-
MNY68_RS16355	94843..94977	+	135	WP_256326490.1	hypothetical protein	-
MNY68_RS16285 (MNY68_16285)	95096..95917	+	822	WP_001029679.1	TnsA endonuclease N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-32 / aph(3')-Ia / sul1 / qacE / ant(3'')-Ia / dfrA1 / lnu(F)	-	1..102837	102837

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T237955 WP_023159957.1 NZ_CP093261:91169-91501 [Moellerella wisconsensis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T237955 NZ_LR883050:208590-208697 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8786.20 Da
• Isoelectric Point: 5.0351

>AT237955 WP_023159984.1 NZ_CP093261:90924-91169 [Moellerella wisconsensis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT237955 NZ_LR883050:c208542-208476 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A2P1BQ75

Antitoxin

• AlphaFold DB: A0A857E7M9