Detailed information of TA system TA237940

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 27462..28170
• Replicon: plasmid pW18-2-a
• Accession: NZ_CP093250
• Organism: Moellerella wisconsensis strain W18-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MNY64_RS16165
• Protein ID: WP_137022559.1
• Coordinates: 27781..28170 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D7IVZ1
• Locus tag: MNY64_RS16160
• Protein ID: WP_067421833.1
• Coordinates: 27462..27791 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY64_RS16125 (MNY64_16130)	23358..23627	+	270	WP_137022510.1	hypothetical protein	-
MNY64_RS16130 (MNY64_16135)	23624..23869	+	246	WP_241502009.1	hypothetical protein	-
MNY64_RS16135 (MNY64_16140)	23984..24328	+	345	WP_137022512.1	hypothetical protein	-
MNY64_RS16140 (MNY64_16145)	24385..24666	+	282	WP_137022513.1	hypothetical protein	-
MNY64_RS16145 (MNY64_16150)	24966..25517	+	552	WP_137022556.1	hypothetical protein	-
MNY64_RS16150 (MNY64_16155)	25605..27254	+	1650	WP_241543886.1	ATP-binding protein	-
MNY64_RS16155 (MNY64_16160)	27241..27423	+	183	WP_137022558.1	hypothetical protein	-
MNY64_RS16160 (MNY64_16165)	27462..27791	-	330	WP_067421833.1	helix-turn-helix domain-containing protein	Antitoxin
MNY64_RS16165 (MNY64_16170)	27781..28170	-	390	WP_137022559.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MNY64_RS16170 (MNY64_16175)	28313..28921	+	609	WP_137022560.1	hypothetical protein	-
MNY64_RS16175 (MNY64_16180)	28975..30195	+	1221	WP_137022561.1	hypothetical protein	-
MNY64_RS16180 (MNY64_16185)	30288..30734	+	447	WP_137022562.1	hypothetical protein	-
MNY64_RS16185 (MNY64_16190)	30779..30919	+	141	WP_175403207.1	hypothetical protein	-
MNY64_RS18360	31134..31256	+	123	WP_277628550.1	hypothetical protein	-
MNY64_RS16190 (MNY64_16200)	31260..32048	+	789	WP_137022564.1	M48 family metallopeptidase	-
MNY64_RS16195 (MNY64_16205)	32116..32793	+	678	WP_137022565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ant(3'')-Ia / lnu(F) / floR / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-1 / mph(A)	-	1..270793	270793

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-93)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14450.56 Da
• Isoelectric Point: 8.4662

>T237940 WP_137022559.1 NZ_CP093250:c28170-27781 [Moellerella wisconsensis]
VAFYKISSFVRSTKKIPITDKQLLDAAHEVQQGQFEADLGGGVIKKRLAMKGQGKSGSVRVIIFFKINNHLFFADGWTKN
TVNAKGTKEIEDDELETYKKLAAEFLNFDGKIINNLISKGILEEITDEQ

Nucleotide

• Length: 390 bp

>T237940 NZ_LR883012:1902689-1902940 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12062.15 Da
• Isoelectric Point: 10.2039

>AT237940 WP_067421833.1 NZ_CP093250:c27791-27462 [Moellerella wisconsensis]
MNNKLNNMLELAKELNAAGASPESLVKDIESTIRAREINDKIKEVSAMSGSEIKMLRTKYCMSQSVLARALGMSKESISK
WERGEKKPSGPALRMLRILESKGPEVLVV

Nucleotide

• Length: 330 bp

>AT237940 NZ_LR883012:1902937-1903191 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A4D7IVZ1