Detailed information of TA system TA237935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 125274..125982
• Replicon: plasmid pW51-a
• Accession: NZ_CP093246
• Organism: Moellerella wisconsensis strain W51

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MNY72_RS16620
• Protein ID: WP_137022559.1
• Coordinates: 125274..125663 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D7IVZ1
• Locus tag: MNY72_RS16625
• Protein ID: WP_067421833.1
• Coordinates: 125653..125982 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY72_RS16585 (MNY72_16580)	120651..121328	-	678	WP_137022565.1	hypothetical protein	-
MNY72_RS16590 (MNY72_16585)	121396..122184	-	789	WP_137022564.1	M48 family metallopeptidase	-
MNY72_RS16595	122188..122349	-	162	WP_241542963.1	hypothetical protein	-
MNY72_RS16600 (MNY72_16590)	122525..122665	-	141	WP_175403207.1	hypothetical protein	-
MNY72_RS16605 (MNY72_16595)	122710..123156	-	447	WP_137022562.1	hypothetical protein	-
MNY72_RS16610 (MNY72_16600)	123249..124469	-	1221	WP_137022561.1	hypothetical protein	-
MNY72_RS16615 (MNY72_16605)	124523..125131	-	609	WP_137022560.1	hypothetical protein	-
MNY72_RS16620 (MNY72_16610)	125274..125663	+	390	WP_137022559.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MNY72_RS16625 (MNY72_16615)	125653..125982	+	330	WP_067421833.1	helix-turn-helix domain-containing protein	Antitoxin
MNY72_RS16630 (MNY72_16620)	126021..126203	-	183	WP_137022558.1	hypothetical protein	-
MNY72_RS16635 (MNY72_16625)	126190..127839	-	1650	WP_137022557.1	ATP-binding protein	-
MNY72_RS16640 (MNY72_16630)	127927..128478	-	552	WP_137022556.1	hypothetical protein	-
MNY72_RS16645 (MNY72_16635)	128778..129059	-	282	WP_137022513.1	hypothetical protein	-
MNY72_RS16650 (MNY72_16640)	129116..129460	-	345	WP_137022512.1	hypothetical protein	-
MNY72_RS16655 (MNY72_16645)	129575..129820	-	246	WP_241502009.1	hypothetical protein	-
MNY72_RS16660 (MNY72_16650)	129817..130086	-	270	WP_241542964.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IIa / aph(3')-Ia / mph(A) / blaCTX-M-1 / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / tet(D)	-	1..270632	270632

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-93)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14450.56 Da
• Isoelectric Point: 8.4662

>T237935 WP_137022559.1 NZ_CP093246:125274-125663 [Moellerella wisconsensis]
VAFYKISSFVRSTKKIPITDKQLLDAAHEVQQGQFEADLGGGVIKKRLAMKGQGKSGSVRVIIFFKINNHLFFADGWTKN
TVNAKGTKEIEDDELETYKKLAAEFLNFDGKIINNLISKGILEEITDEQ

Nucleotide

• Length: 390 bp

>T237935 NZ_LR883012:c632289-631825 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12062.15 Da
• Isoelectric Point: 10.2039

>AT237935 WP_067421833.1 NZ_CP093246:125653-125982 [Moellerella wisconsensis]
MNNKLNNMLELAKELNAAGASPESLVKDIESTIRAREINDKIKEVSAMSGSEIKMLRTKYCMSQSVLARALGMSKESISK
WERGEKKPSGPALRMLRILESKGPEVLVV

Nucleotide

• Length: 330 bp

>AT237935 NZ_LR883012:c632624-632289 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCTCTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A4D7IVZ1