Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 292893..293470 Replicon plasmid pW65-a
Accession NZ_CP093243
Organism Moellerella wisconsensis strain W65

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag MNY66_RS17635 Protein ID WP_023159957.1
Coordinates 292893..293225 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U5N340
Locus tag MNY66_RS17640 Protein ID WP_023159984.1
Coordinates 293225..293470 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY66_RS17960 288735..288869 - 135 WP_256326490.1 hypothetical protein -
MNY66_RS17605 (MNY66_17605) 288885..289838 - 954 WP_048821757.1 site-specific integrase -
MNY66_RS17610 (MNY66_17610) 290324..291004 + 681 Protein_375 type I restriction endonuclease -
MNY66_RS17615 (MNY66_17615) 291085..291222 + 138 Protein_376 HdeA/HdeB family chaperone -
MNY66_RS17620 (MNY66_17620) 291230..291877 + 648 WP_241501931.1 GAD-like domain-containing protein -
MNY66_RS17625 (MNY66_17625) 291893..292522 + 630 WP_241501932.1 GAD-like domain-containing protein -
MNY66_RS17630 (MNY66_17630) 292589..292918 + 330 WP_241501933.1 SMI1/KNR4 family protein -
MNY66_RS17635 (MNY66_17635) 292893..293225 - 333 WP_023159957.1 endoribonuclease MazF Toxin
MNY66_RS17640 (MNY66_17640) 293225..293470 - 246 WP_023159984.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
MNY66_RS17645 (MNY66_17645) 293721..294344 + 624 WP_023159958.1 AAA family ATPase -
MNY66_RS17650 (MNY66_17650) 294461..294688 + 228 WP_048821767.1 plasmid partition protein ParG -
MNY66_RS17655 (MNY66_17655) 294780..295541 - 762 WP_241501934.1 IS5 family transposase -
MNY66_RS17660 (MNY66_17660) 295659..296501 + 843 WP_241501935.1 hypothetical protein -
MNY66_RS17665 (MNY66_17665) 296910..298175 + 1266 WP_241501936.1 integrase arm-type DNA-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(H) / aadA14 / dfrA1 / ant(3'')-Ia / sul3 / blaCTX-M-1 / mph(A) / aph(3')-Ia - 1..345391 345391


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T237928 WP_023159957.1 NZ_CP093243:c293225-292893 [Moellerella wisconsensis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T237928 NZ_LR883008:2061-2348 [Escherichia coli]
ATGACTTATACGGTTAAATTCAGGGATGATGCCCTCAGGGAATGGCTGAAACTGGATAAATCCGTTCAACAACAGTTCGC
AAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCTTCAGCAAAACTACGTGGATTAAAAGATTGCTACAAAATAA
AACTTCGCGCATCAGGTTTTCGTCTGGTCTATCAGGTGATTGATGACATGTTAATTATTGCGGTTGTCGCAGTAGGTAAA
CGTGAACGTAGTCATGTTTACAATCTCGCCAGCGAGAGAATGAGATAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8786.20 Da        Isoelectric Point: 5.0351

>AT237928 WP_023159984.1 NZ_CP093243:c293470-293225 [Moellerella wisconsensis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT237928 NZ_LR883008:1813-2064 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAACTGAAAAAAAATCCAATGGCGACAGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCTGCATTCTACTGTGTTCCTGCTGAATTGTATGAAAGGATGC
TTGATGCTATGGATGACCAGGAGCTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTACATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75

Antitoxin

Source ID Structure
AlphaFold DB A0A857E7M9

References