Detailed information of TA system TA237924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 129138..129846
• Replicon: plasmid pW65-a
• Accession: NZ_CP093243
• Organism: Moellerella wisconsensis strain W65

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: MNY66_RS16610
• Protein ID: WP_241502024.1
• Coordinates: 129457..129846 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A4D7IVZ1
• Locus tag: MNY66_RS16605
• Protein ID: WP_067421833.1
• Coordinates: 129138..129467 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY66_RS16580 (MNY66_16580)	124874..125155	+	282	WP_137022554.1	hypothetical protein	-
MNY66_RS16585 (MNY66_16585)	125369..126433	+	1065	WP_241502022.1	hypothetical protein	-
MNY66_RS16590 (MNY66_16590)	126642..127193	+	552	WP_137022556.1	hypothetical protein	-
MNY66_RS16595 (MNY66_16595)	127281..128930	+	1650	WP_241502023.1	ATP-binding protein	-
MNY66_RS16600 (MNY66_16600)	128917..129099	+	183	WP_137022558.1	hypothetical protein	-
MNY66_RS16605 (MNY66_16605)	129138..129467	-	330	WP_067421833.1	helix-turn-helix domain-containing protein	Antitoxin
MNY66_RS16610 (MNY66_16610)	129457..129846	-	390	WP_241502024.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MNY66_RS16615 (MNY66_16615)	129989..130597	+	609	WP_137022560.1	hypothetical protein	-
MNY66_RS16620 (MNY66_16620)	130651..131871	+	1221	WP_137022561.1	hypothetical protein	-
MNY66_RS16625 (MNY66_16625)	131964..132410	+	447	WP_137022562.1	hypothetical protein	-
MNY66_RS16630 (MNY66_16630)	132455..132595	+	141	WP_175403207.1	hypothetical protein	-
MNY66_RS16635 (MNY66_16635)	132630..132944	+	315	WP_241502025.1	hypothetical protein	-
MNY66_RS16640 (MNY66_16640)	132948..133736	+	789	WP_137022564.1	M48 family metallopeptidase	-
MNY66_RS16645 (MNY66_16645)	133804..134481	+	678	WP_137022565.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(H) / aadA14 / dfrA1 / ant(3'')-Ia / sul3 / blaCTX-M-1 / mph(A) / aph(3')-Ia	-	1..345391	345391

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-93)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14451.50 Da
• Isoelectric Point: 6.4817

>T237924 WP_241502024.1 NZ_CP093243:c129846-129457 [Moellerella wisconsensis]
VAFYKISSFVRSTKKIPITDKQLLDAAHEVQQGQFEADLGGGVIKKRLAMKGQGKSGSVRVIIFFKINNHLFFADGWTKN
TVNAKGTKEIEDDELETYKKLAAEFLNFDGEIINNLISKGILEEITDEQ

Nucleotide

• Length: 390 bp

>T237924 NZ_LR883006:4251669-4252010 [Escherichia coli]
ATGACTGACACGTATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCTCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGCCGCTACCCGGATCACGCGCATATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGCATCGTCCTCACCGCTCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCGCTGCGCAGGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGAATTTATTGGGGTGATTGC
CGGTAAACAGAAAGTTGCCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12062.15 Da
• Isoelectric Point: 10.2039

>AT237924 WP_067421833.1 NZ_CP093243:c129467-129138 [Moellerella wisconsensis]
MNNKLNNMLELAKELNAAGASPESLVKDIESTIRAREINDKIKEVSAMSGSEIKMLRTKYCMSQSVLARALGMSKESISK
WERGEKKPSGPALRMLRILESKGPEVLVV

Nucleotide

• Length: 330 bp

>AT237924 NZ_LR883006:c4251674-4251598 [Escherichia coli]
AGTCATAACTGCTATTCTCCGATAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin

• AlphaFold DB: A0A4D7IVZ1