Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4148101..4148879 Replicon chromosome
Accession NZ_CP093239
Organism Escherichia marmotae strain W49-2

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag MNY74_RS20230 Protein ID WP_001517064.1
Coordinates 4148101..4148589 (-) Length 163 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A7H9KCB5
Locus tag MNY74_RS20235 Protein ID WP_001110459.1
Coordinates 4148586..4148879 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY74_RS20195 (4143180) 4143180..4143554 + 375 WP_053272399.1 type IV toxin-antitoxin system YeeU family antitoxin -
MNY74_RS20200 (4143600) 4143600..4143977 + 378 WP_053272398.1 TA system toxin CbtA family protein -
MNY74_RS20205 (4143974) 4143974..4144462 + 489 WP_187194217.1 DUF5983 family protein -
MNY74_RS20210 (4144474) 4144474..4144671 + 198 WP_000839250.1 DUF957 domain-containing protein -
MNY74_RS20215 (4144756) 4144756..4145600 + 845 Protein_3970 DUF4942 domain-containing protein -
MNY74_RS20220 (4145671) 4145671..4147206 + 1536 WP_061362221.1 EAL domain-containing protein -
MNY74_RS20225 (4147230) 4147230..4147520 + 291 WP_241556745.1 hypothetical protein -
MNY74_RS20230 (4148101) 4148101..4148589 - 489 WP_001517064.1 GNAT family N-acetyltransferase Toxin
MNY74_RS20235 (4148586) 4148586..4148879 - 294 WP_001110459.1 DUF1778 domain-containing protein Antitoxin
MNY74_RS20240 (4149414) 4149414..4149911 - 498 WP_016249187.1 type VI secretion system contractile sheath small subunit -
MNY74_RS20245 (4150503) 4150503..4150976 + 474 WP_001517065.1 hypothetical protein -
MNY74_RS20250 (4150973) 4150973..4151308 + 336 WP_181478621.1 hypothetical protein -
MNY74_RS20255 (4152134) 4152134..4153117 + 984 WP_001325875.1 KpsF/GutQ family sugar-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - clpV/tssH / hcp/tssD / vipB/tssC / vipA/tssB 3994242..4149129 154887


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 163 a.a.        Molecular weight: 17521.41 Da        Isoelectric Point: 7.7535

>T237917 WP_001517064.1 NZ_CP093239:c4148589-4148101 [Escherichia marmotae]
MISAPEPLHAGHILTPFCCGVDSMDNWLKQRAMKNQVTGASRTFVCCGSDSKVLAYYSLASSAVMTNTAPGRFRRNMPDP
IPVVVLGRLAVDQSIHGQGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLLVTLGDLV
HA

Download         Length: 489 bp

>T237917 NZ_LR883006:2787264-2787371 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10949.56 Da        Isoelectric Point: 9.9171

>AT237917 WP_001110459.1 NZ_CP093239:c4148879-4148586 [Escherichia marmotae]
MPAANSMAMKRETLNLRIRPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQTFLTRLDQAPAPN
SALRKTMQTPAPWEQEK

Download         Length: 294 bp

>AT237917 NZ_LR883006:c2787216-2787149 [Escherichia coli]
GTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A7H9KCB5

References