Detailed information of TA system TA237916

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4143180..4143977
• Replicon: chromosome
• Accession: NZ_CP093239
• Organism: Escherichia marmotae strain W49-2

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A6D0I2J3
• Locus tag: MNY74_RS20200
• Protein ID: WP_053272398.1
• Coordinates: 4143600..4143977 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7A2X194
• Locus tag: MNY74_RS20195
• Protein ID: WP_053272399.1
• Coordinates: 4143180..4143554 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY74_RS20160 (4138935)	4138935..4139819	+	885	WP_089674944.1	50S ribosome-binding GTPase	-
MNY74_RS20165 (4139938)	4139938..4140615	+	678	WP_097291264.1	hypothetical protein	-
MNY74_RS20170 (4140621)	4140621..4140854	+	234	WP_053272404.1	DUF905 family protein	-
MNY74_RS20175 (4140944)	4140944..4141762	+	819	WP_053272403.1	DUF932 domain-containing protein	-
MNY74_RS20180 (4141839)	4141839..4142324	+	486	WP_053272402.1	antirestriction protein	-
MNY74_RS20185 (4142339)	4142339..4142815	+	477	WP_053272401.1	RadC family protein	-
MNY74_RS20190 (4142884)	4142884..4143105	+	222	WP_000692345.1	DUF987 domain-containing protein	-
MNY74_RS20195 (4143180)	4143180..4143554	+	375	WP_053272399.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MNY74_RS20200 (4143600)	4143600..4143977	+	378	WP_053272398.1	TA system toxin CbtA family protein	Toxin
MNY74_RS20205 (4143974)	4143974..4144462	+	489	WP_187194217.1	DUF5983 family protein	-
MNY74_RS20210 (4144474)	4144474..4144671	+	198	WP_000839250.1	DUF957 domain-containing protein	-
MNY74_RS20215 (4144756)	4144756..4145600	+	845	Protein_3970	DUF4942 domain-containing protein	-
MNY74_RS20220 (4145671)	4145671..4147206	+	1536	WP_061362221.1	EAL domain-containing protein	-
MNY74_RS20225 (4147230)	4147230..4147520	+	291	WP_241556745.1	hypothetical protein	-
MNY74_RS20230 (4148101)	4148101..4148589	-	489	WP_001517064.1	GNAT family N-acetyltransferase	-
MNY74_RS20235 (4148586)	4148586..4148879	-	294	WP_001110459.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	clpV/tssH / hcp/tssD / vipB/tssC / vipA/tssB	3994242..4149129	154887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14091.10 Da
• Isoelectric Point: 8.2904

>T237916 WP_053272398.1 NZ_CP093239:4143600-4143977 [Escherichia marmotae]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T237916 NZ_LR883006:c2735432-2735277 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13826.60 Da
• Isoelectric Point: 6.4761

>AT237916 WP_053272399.1 NZ_CP093239:4143180-4143554 [Escherichia marmotae]
VSDTLHETTHPDDNRGRPWWGLPCTVTPCFGARMVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT237916 NZ_LR883006:2735444-2735502 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A6D0I2J3

Antitoxin

• AlphaFold DB: A0A7A2X194