Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 397045..397541 Replicon chromosome
Accession NZ_CP093239
Organism Escherichia marmotae strain W49-2

Toxin (Protein)


Gene name ghoT Uniprot ID A0A7U9DCZ4
Locus tag MNY74_RS01775 Protein ID WP_001173345.1
Coordinates 397368..397541 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A7U9DAJ1
Locus tag MNY74_RS01770 Protein ID WP_001517605.1
Coordinates 397045..397341 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY74_RS01750 (393777) 393777..394496 - 720 WP_000611294.1 two-component system response regulator DcuR -
MNY74_RS01755 (394493) 394493..396124 - 1632 WP_038355161.1 sensor histidine kinase -
MNY74_RS01760 (396303) 396303..396533 + 231 WP_000371696.1 4Fe-4S mono-cluster protein YjdI -
MNY74_RS01765 (396545) 396545..396817 + 273 WP_000405652.1 GNAT family N-acetyltransferase -
MNY74_RS01770 (397045) 397045..397341 + 297 WP_001517605.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MNY74_RS01775 (397368) 397368..397541 + 174 WP_001173345.1 type V toxin-antitoxin system toxin GhoT Toxin
MNY74_RS01780 (397660) 397660..399177 - 1518 WP_001295074.1 lysine--tRNA ligase -
MNY74_RS01785 (399414) 399414..400871 - 1458 WP_001517606.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 397045..406393 9348


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(7-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T237890 WP_001173345.1 NZ_CP093239:397368-397541 [Escherichia marmotae]
MALFSKILIFYVIGVNISFVIIWFLSHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T237890 NZ_LR882997:4091650-4091928 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11568.18 Da        Isoelectric Point: 4.0994

>AT237890 WP_001517605.1 NZ_CP093239:397045-397341 [Escherichia marmotae]
MEGINRFKTYVVSFDYPSSYYSVFLRLRSLMYDMDFSSIVADEYGIPRQLNENAFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMIVDDYFRQFYK

Download         Length: 297 bp

>AT237890 NZ_LR882997:4091387-4091647 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9DCZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DAJ1

References