Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 68317..68918 Replicon plasmid pEC-16-35-3
Accession NZ_CP093228
Organism Escherichia coli strain EC-16-35

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag MNY24_RS24670 Protein ID WP_001216045.1
Coordinates 68317..68697 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag MNY24_RS24675 Protein ID WP_001190712.1
Coordinates 68697..68918 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY24_RS24645 63757..65199 - 1443 WP_224114847.1 terminase -
MNY24_RS24650 65241..66434 - 1194 WP_000219604.1 terminase -
MNY24_RS24655 66521..66973 - 453 WP_001369802.1 hypothetical protein -
MNY24_RS24660 67062..68105 - 1044 WP_000648825.1 DUF968 domain-containing protein -
MNY24_RS24665 68133..68312 - 180 WP_023153731.1 hypothetical protein -
MNY24_RS24670 68317..68697 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MNY24_RS24675 68697..68918 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MNY24_RS24680 68991..69380 - 390 WP_000506726.1 S24 family peptidase -
MNY24_RS24685 69504..69755 - 252 WP_001377386.1 DNA polymerase III subunit theta -
MNY24_RS24690 70205..70342 + 138 WP_000123562.1 hypothetical protein -
MNY24_RS24695 70417..70779 - 363 WP_001261544.1 hypothetical protein -
MNY24_RS24700 70776..71708 - 933 WP_000057449.1 hypothetical protein -
MNY24_RS24705 71690..72064 - 375 WP_000988658.1 hypothetical protein -
MNY24_RS24710 72071..72364 - 294 WP_001677496.1 hypothetical protein -
MNY24_RS24715 72543..72776 - 234 WP_000516537.1 hypothetical protein -
MNY24_RS25975 72859..73155 - 297 WP_224690240.1 DUF551 domain-containing protein -
MNY24_RS24725 73423..73602 - 180 Protein_73 hypothetical protein -
MNY24_RS24730 73618..73746 - 129 Protein_74 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..99719 99719


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T237886 WP_001216045.1 NZ_CP093228:c68697-68317 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T237886 NZ_LR882997:2956929-2957036 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT237886 WP_001190712.1 NZ_CP093228:c68918-68697 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT237886 NZ_LR882997:c2956876-2956815 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References