Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 103480..104005 Replicon plasmid pEC-16-35-2
Accession NZ_CP093227
Organism Escherichia coli strain EC-16-35

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag MNY24_RS23685 Protein ID WP_001159871.1
Coordinates 103700..104005 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag MNY24_RS23680 Protein ID WP_000813630.1
Coordinates 103480..103698 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY24_RS23655 (98679) 98679..99376 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
MNY24_RS23660 (99630) 99630..100652 - 1023 WP_000361402.1 helicase UvrD -
MNY24_RS23665 (100637) 100637..102202 - 1566 WP_001128474.1 AAA family ATPase -
MNY24_RS23670 (102277) 102277..102693 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
MNY24_RS23675 (102690) 102690..102920 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
MNY24_RS23680 (103480) 103480..103698 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
MNY24_RS23685 (103700) 103700..104005 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
MNY24_RS23690 (104006) 104006..104812 + 807 WP_000016970.1 site-specific integrase -
MNY24_RS23695 (105534) 105534..106289 + 756 WP_000852146.1 replication initiation protein RepE -
MNY24_RS23700 (106877) 106877..108043 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA17 / aadA5 / qacE / sul1 / mph(A) / floR / aph(6)-Id / aph(3'')-Ib / sul2 / erm(B) / blaTEM-1B / rmtB / fosA3 / blaCTX-M-55 - 1..212822 212822


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T237882 WP_001159871.1 NZ_CP093227:103700-104005 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T237882 NZ_LR882997:c1996848-1996474 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT237882 WP_000813630.1 NZ_CP093227:103480-103698 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT237882 NZ_LR882997:c1997306-1996938 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References