Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 102277..102920 Replicon plasmid pEC-16-35-2
Accession NZ_CP093227
Organism Escherichia coli strain EC-16-35

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag MNY24_RS23670 Protein ID WP_001034044.1
Coordinates 102277..102693 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag MNY24_RS23675 Protein ID WP_001261286.1
Coordinates 102690..102920 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY24_RS23645 (97519) 97519..97632 - 114 Protein_112 IS6 family transposase -
MNY24_RS23650 (97832) 97832..98529 + 698 WP_223350503.1 IS1 family transposase -
MNY24_RS23655 (98679) 98679..99376 + 698 WP_103215986.1 IS1-like element IS1A family transposase -
MNY24_RS23660 (99630) 99630..100652 - 1023 WP_000361402.1 helicase UvrD -
MNY24_RS23665 (100637) 100637..102202 - 1566 WP_001128474.1 AAA family ATPase -
MNY24_RS23670 (102277) 102277..102693 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
MNY24_RS23675 (102690) 102690..102920 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MNY24_RS23680 (103480) 103480..103698 + 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
MNY24_RS23685 (103700) 103700..104005 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
MNY24_RS23690 (104006) 104006..104812 + 807 WP_000016970.1 site-specific integrase -
MNY24_RS23695 (105534) 105534..106289 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA17 / aadA5 / qacE / sul1 / mph(A) / floR / aph(6)-Id / aph(3'')-Ib / sul2 / erm(B) / blaTEM-1B / rmtB / fosA3 / blaCTX-M-55 - 1..212822 212822


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T237881 WP_001034044.1 NZ_CP093227:c102693-102277 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T237881 NZ_LR882997:1960301-1960555 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAATCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT237881 WP_001261286.1 NZ_CP093227:c102920-102690 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT237881 NZ_LR882997:1960053-1960304 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References