Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4274721..4275218 Replicon chromosome
Accession NZ_CP093226
Organism Escherichia coli strain EC-16-35

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MNY24_RS20640 Protein ID WP_001173343.1
Coordinates 4274721..4274894 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MNY24_RS20645 Protein ID WP_000398619.1
Coordinates 4274922..4275218 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY24_RS20630 (4271391) 4271391..4272848 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
MNY24_RS20635 (4273085) 4273085..4274602 + 1518 WP_001295074.1 lysine--tRNA ligase -
MNY24_RS20640 (4274721) 4274721..4274894 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MNY24_RS20645 (4274922) 4274922..4275218 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MNY24_RS20650 (4275445) 4275445..4275717 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
MNY24_RS20655 (4275729) 4275729..4275959 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MNY24_RS20660 (4276140) 4276140..4277771 + 1632 WP_001216477.1 sensor histidine kinase -
MNY24_RS20665 (4277768) 4277768..4278487 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T237873 WP_001173343.1 NZ_CP093226:c4274894-4274721 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T237873 NZ_LR882991:100204-100410 [Escherichia coli]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACCTTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT237873 WP_000398619.1 NZ_CP093226:c4275218-4274922 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT237873 NZ_LR882991:c100212-100156 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCCTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References