Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3474763..3475381 Replicon chromosome
Accession NZ_CP093226
Organism Escherichia coli strain EC-16-35

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MNY24_RS16700 Protein ID WP_001291435.1
Coordinates 3475163..3475381 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MNY24_RS16695 Protein ID WP_000344800.1
Coordinates 3474763..3475137 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNY24_RS16685 (3469852) 3469852..3471045 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MNY24_RS16690 (3471068) 3471068..3474217 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MNY24_RS16695 (3474763) 3474763..3475137 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MNY24_RS16700 (3475163) 3475163..3475381 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MNY24_RS16705 (3475553) 3475553..3476104 + 552 WP_000102564.1 maltose O-acetyltransferase -
MNY24_RS16710 (3476220) 3476220..3476690 + 471 WP_000136192.1 YlaC family protein -
MNY24_RS16715 (3476854) 3476854..3478404 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MNY24_RS16720 (3478446) 3478446..3478799 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MNY24_RS16730 (3479178) 3479178..3479489 + 312 WP_000409911.1 MGMT family protein -
MNY24_RS16735 (3479520) 3479520..3480092 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T237868 WP_001291435.1 NZ_CP093226:3475163-3475381 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T237868 NZ_LR882990:2768572-2768679 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT237868 WP_000344800.1 NZ_CP093226:3474763-3475137 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT237868 NZ_LR882990:c2768525-2768459 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References