Detailed information of TA system TA237867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2442209..2442847
• Replicon: chromosome
• Accession: NZ_CP093226
• Organism: Escherichia coli strain EC-16-35

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: MNY24_RS11690
• Protein ID: WP_001447010.1
• Coordinates: 2442671..2442847 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MNY24_RS11685
• Protein ID: WP_001270286.1
• Coordinates: 2442209..2442625 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNY24_RS11665 (2437361)	2437361..2438302	-	942	WP_001251304.1	ABC transporter permease	-
MNY24_RS11670 (2438303)	2438303..2439316	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
MNY24_RS11675 (2439334)	2439334..2440479	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
MNY24_RS11680 (2440724)	2440724..2442130	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
MNY24_RS11685 (2442209)	2442209..2442625	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MNY24_RS11690 (2442671)	2442671..2442847	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MNY24_RS11695 (2443069)	2443069..2443299	+	231	WP_000494244.1	YncJ family protein	-
MNY24_RS11700 (2443391)	2443391..2445352	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MNY24_RS11705 (2445425)	2445425..2445961	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
MNY24_RS11710 (2446053)	2446053..2447228	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T237867 WP_001447010.1 NZ_CP093226:c2442847-2442671 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T237867 NZ_LR882990:c2510755-2510579 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAAGTCAG
GTTTCATGGGATGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCAGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT237867 WP_001270286.1 NZ_CP093226:c2442625-2442209 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT237867 NZ_LR882990:c2510533-2510117 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCATTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGAGAATTGGCAAACCTAAACAGGAAAT
TACTCGTTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8