Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2923428..2924259 Replicon chromosome
Accession NZ_CP093221
Organism Escherichia coli strain ER2683

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag MNV48_RS14010 Protein ID WP_000854814.1
Coordinates 2923428..2923802 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag MNV48_RS14015 Protein ID WP_001285584.1
Coordinates 2923891..2924259 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNV48_RS13970 (2918824) 2918824..2919990 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
MNV48_RS13975 (2920109) 2920109..2920582 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
MNV48_RS13980 (2920780) 2920780..2921838 + 1059 WP_001200891.1 FUSC family protein -
MNV48_RS13985 (2922010) 2922010..2922339 + 330 WP_000450409.1 DUF496 family protein -
MNV48_RS13990 (2922440) 2922440..2922574 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
MNV48_RS13995 (2922694) 2922694..2922822 + 129 Protein_2731 transposase domain-containing protein -
MNV48_RS14000 (2923111) 2923111..2923191 - 81 Protein_2732 hypothetical protein -
MNV48_RS14005 (2923237) 2923237..2923431 - 195 WP_000988600.1 DUF5983 family protein -
MNV48_RS14010 (2923428) 2923428..2923802 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
MNV48_RS14015 (2923891) 2923891..2924259 - 369 WP_001285584.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
MNV48_RS14020 (2924333) 2924333..2924554 - 222 WP_000692323.1 DUF987 domain-containing protein -
MNV48_RS14025 (2924617) 2924617..2925063 - 447 WP_000187523.1 RadC family protein -
MNV48_RS14030 (2925060) 2925060..2926592 - 1533 WP_001350525.1 protein YeeR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T237832 WP_000854814.1 NZ_CP093221:c2923802-2923428 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T237832 NZ_LR882974:c33708-33502 [Escherichia coli]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATACCCTTATTGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTTTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT237832 WP_001285584.1 NZ_CP093221:c2924259-2923891 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT237832 NZ_LR882974:33700-33756 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References