Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1964635..1965289 Replicon chromosome
Accession NZ_CP093221
Organism Escherichia coli strain ER2683

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag MNV48_RS09530 Protein ID WP_000244777.1
Coordinates 1964882..1965289 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag MNV48_RS09525 Protein ID WP_000354046.1
Coordinates 1964635..1964901 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNV48_RS09500 (1959804) 1959804..1960547 + 744 WP_000951964.1 SDR family oxidoreductase -
MNV48_RS09505 (1960604) 1960604..1962037 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
MNV48_RS09510 (1962082) 1962082..1962393 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
MNV48_RS09515 (1962557) 1962557..1963216 + 660 WP_000250274.1 hemolysin III family protein -
MNV48_RS09520 (1963412) 1963412..1964392 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
MNV48_RS09525 (1964635) 1964635..1964901 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
MNV48_RS09530 (1964882) 1964882..1965289 + 408 WP_000244777.1 protein YgfX Toxin
MNV48_RS09535 (1965329) 1965329..1965850 - 522 WP_001055874.1 flavodoxin FldB -
MNV48_RS09540 (1965962) 1965962..1966858 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MNV48_RS09545 (1966883) 1966883..1967593 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MNV48_RS09550 (1967599) 1967599..1969332 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T237827 WP_000244777.1 NZ_CP093221:1964882-1965289 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T237827 NZ_LR882973:2656152-2656259 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT237827 WP_000354046.1 NZ_CP093221:1964635-1964901 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT237827 NZ_LR882973:c2656105-2656039 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References