Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 651397..651894 Replicon chromosome
Accession NZ_CP093221
Organism Escherichia coli strain ER2683

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MNV48_RS03280 Protein ID WP_001173343.1
Coordinates 651397..651570 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag MNV48_RS03285 Protein ID WP_000398619.1
Coordinates 651598..651894 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNV48_RS03270 (648067) 648067..649524 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
MNV48_RS03275 (649761) 649761..651278 + 1518 WP_001295074.1 lysine--tRNA ligase -
MNV48_RS03280 (651397) 651397..651570 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MNV48_RS03285 (651598) 651598..651894 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MNV48_RS03290 (652121) 652121..652393 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
MNV48_RS03295 (652405) 652405..652635 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
MNV48_RS03300 (652816) 652816..654447 + 1632 WP_001216456.1 sensor histidine kinase -
MNV48_RS03305 (654444) 654444..655163 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T237823 WP_001173343.1 NZ_CP093221:c651570-651397 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T237823 NZ_LR882973:2048421-2048524 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT237823 WP_000398619.1 NZ_CP093221:c651894-651598 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT237823 NZ_LR882973:c2048562-2048291 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References