Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 886289..886818 Replicon chromosome
Accession NZ_CP093188
Organism Staphylococcus epidermidis strain TMDU-302

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag MNU45_RS04090 Protein ID WP_001829891.1
Coordinates 886456..886818 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag MNU45_RS04085 Protein ID WP_001829931.1
Coordinates 886289..886459 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNU45_RS04060 (MNU45_04055) 882114..882593 + 480 WP_001829909.1 PH domain-containing protein -
MNU45_RS04065 (MNU45_04060) 882586..884091 + 1506 WP_001829976.1 PH domain-containing protein -
MNU45_RS04070 (MNU45_04065) 884078..884587 + 510 WP_001829888.1 PH domain-containing protein -
MNU45_RS04075 (MNU45_04070) 884635..884988 + 354 WP_001829915.1 holo-ACP synthase -
MNU45_RS04080 (MNU45_04075) 885054..886202 + 1149 WP_001829972.1 alanine racemase -
MNU45_RS04085 (MNU45_04080) 886289..886459 + 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MNU45_RS04090 (MNU45_04085) 886456..886818 + 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MNU45_RS04095 (MNU45_04090) 887164..888165 + 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
MNU45_RS04100 (MNU45_04095) 888265..888591 + 327 WP_001829952.1 anti-sigma factor antagonist -
MNU45_RS04105 (MNU45_04100) 888593..889072 + 480 WP_001829903.1 anti-sigma B factor RsbW -
MNU45_RS04110 (MNU45_04105) 889047..889817 + 771 WP_002440602.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T237768 WP_001829891.1 NZ_CP093188:886456-886818 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T237768 NZ_LR882500:3815769-3816179 [Mycobacterium tuberculosis variant microti]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT237768 WP_001829931.1 NZ_CP093188:886289-886459 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT237768 NZ_LR882500:3815494-3815772 [Mycobacterium tuberculosis variant microti]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References