Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 878074..878603 Replicon chromosome
Accession NZ_CP093185
Organism Staphylococcus epidermidis strain TMDU-300

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag MNU53_RS04055 Protein ID WP_001829891.1
Coordinates 878241..878603 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag MNU53_RS04050 Protein ID WP_001829931.1
Coordinates 878074..878244 (+) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNU53_RS04025 (MNU53_04020) 873898..874377 + 480 WP_002457111.1 PH domain-containing protein -
MNU53_RS04030 (MNU53_04025) 874370..875875 + 1506 WP_002487879.1 PH domain-containing protein -
MNU53_RS04035 (MNU53_04030) 875862..876371 + 510 WP_002457109.1 PH domain-containing protein -
MNU53_RS04040 (MNU53_04035) 876419..876772 + 354 WP_002457108.1 holo-ACP synthase -
MNU53_RS04045 (MNU53_04040) 876839..877987 + 1149 WP_002457107.1 alanine racemase -
MNU53_RS04050 (MNU53_04045) 878074..878244 + 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MNU53_RS04055 (MNU53_04050) 878241..878603 + 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MNU53_RS04060 (MNU53_04055) 878948..879949 + 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
MNU53_RS04065 (MNU53_04060) 880049..880375 + 327 WP_001829952.1 anti-sigma factor antagonist -
MNU53_RS04070 (MNU53_04065) 880377..880856 + 480 WP_002487882.1 anti-sigma B factor RsbW -
MNU53_RS04075 (MNU53_04070) 880831..881601 + 771 WP_002440602.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T237764 WP_001829891.1 NZ_CP093185:878241-878603 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T237764 NZ_LR882500:3554718-3555062 [Mycobacterium tuberculosis variant microti]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT237764 WP_001829931.1 NZ_CP093185:878074-878244 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT237764 NZ_LR882500:3555059-3555388 [Mycobacterium tuberculosis variant microti]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References