Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1879391..1879920 Replicon chromosome
Accession NZ_CP093181
Organism Staphylococcus epidermidis strain TMDU-265

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag MNU40_RS09320 Protein ID WP_001829891.1
Coordinates 1879391..1879753 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag MNU40_RS09325 Protein ID WP_001829931.1
Coordinates 1879750..1879920 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNU40_RS09300 (MNU40_09305) 1876392..1877162 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
MNU40_RS09305 (MNU40_09310) 1877137..1877616 - 480 WP_001829903.1 anti-sigma B factor RsbW -
MNU40_RS09310 (MNU40_09315) 1877618..1877944 - 327 WP_001829952.1 anti-sigma factor antagonist -
MNU40_RS09315 (MNU40_09320) 1878044..1879045 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
MNU40_RS09320 (MNU40_09325) 1879391..1879753 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MNU40_RS09325 (MNU40_09330) 1879750..1879920 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MNU40_RS09330 (MNU40_09335) 1880007..1881155 - 1149 WP_002468951.1 alanine racemase -
MNU40_RS09335 (MNU40_09340) 1881221..1881574 - 354 WP_001829915.1 holo-ACP synthase -
MNU40_RS09340 (MNU40_09345) 1881622..1882131 - 510 WP_001829888.1 PH domain-containing protein -
MNU40_RS09345 (MNU40_09350) 1882118..1883623 - 1506 WP_001829976.1 PH domain-containing protein -
MNU40_RS09350 (MNU40_09355) 1883616..1884095 - 480 WP_001829909.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T237761 WP_001829891.1 NZ_CP093181:c1879753-1879391 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T237761 NZ_LR882500:c3136411-3136019 [Mycobacterium tuberculosis variant microti]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT237761 WP_001829931.1 NZ_CP093181:c1879920-1879750 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT237761 NZ_LR882500:c3136623-3136408 [Mycobacterium tuberculosis variant microti]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References