Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1933040..1933569 Replicon chromosome
Accession NZ_CP093179
Organism Staphylococcus epidermidis strain TMDU-190

Toxin (Protein)


Gene name mazF Uniprot ID Q5HME7
Locus tag MNU31_RS09585 Protein ID WP_001829891.1
Coordinates 1933040..1933402 (-) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q5HME6
Locus tag MNU31_RS09590 Protein ID WP_001829931.1
Coordinates 1933399..1933569 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MNU31_RS09565 (MNU31_09570) 1930041..1930811 - 771 WP_002468952.1 RNA polymerase sigma factor SigB -
MNU31_RS09570 (MNU31_09575) 1930786..1931265 - 480 WP_001829903.1 anti-sigma B factor RsbW -
MNU31_RS09575 (MNU31_09580) 1931267..1931593 - 327 WP_001829952.1 anti-sigma factor antagonist -
MNU31_RS09580 (MNU31_09585) 1931693..1932694 - 1002 WP_001829902.1 PP2C family protein-serine/threonine phosphatase -
MNU31_RS09585 (MNU31_09590) 1933040..1933402 - 363 WP_001829891.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MNU31_RS09590 (MNU31_09595) 1933399..1933569 - 171 WP_001829931.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MNU31_RS09595 (MNU31_09600) 1933656..1934804 - 1149 WP_002468951.1 alanine racemase -
MNU31_RS09600 (MNU31_09605) 1934870..1935223 - 354 WP_001829915.1 holo-ACP synthase -
MNU31_RS09605 (MNU31_09610) 1935271..1935780 - 510 WP_001829888.1 PH domain-containing protein -
MNU31_RS09610 (MNU31_09615) 1935767..1937272 - 1506 WP_001829976.1 PH domain-containing protein -
MNU31_RS09615 (MNU31_09620) 1937265..1937744 - 480 WP_001829909.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13497.65 Da        Isoelectric Point: 9.9522

>T237755 WP_001829891.1 NZ_CP093179:c1933402-1933040 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSESKMIEVDNALDISLGLNNFDHHKS

Download         Length: 363 bp

>T237755 NZ_LR882500:2933842-2934282 [Mycobacterium tuberculosis variant microti]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6289.92 Da        Isoelectric Point: 4.1920

>AT237755 WP_001829931.1 NZ_CP093179:c1933569-1933399 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Download         Length: 171 bp

>AT237755 NZ_LR882500:2933568-2933855 [Mycobacterium tuberculosis variant microti]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2G7HWR0

Antitoxin

Source ID Structure
AlphaFold DB A0A0N1EF65

References