Detailed information of TA system TA237743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 889818..890347
• Replicon: chromosome
• Accession: NZ_CP093170
• Organism: Staphylococcus epidermidis strain TMDU-128

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A939SRT1
• Locus tag: MNU35_RS04165
• Protein ID: WP_002447757.1
• Coordinates: 889985..890347 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q5HME6
• Locus tag: MNU35_RS04160
• Protein ID: WP_001829931.1
• Coordinates: 889818..889988 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNU35_RS04135 (MNU35_04140)	885642..886121	+	480	WP_002447762.1	PH domain-containing protein	-
MNU35_RS04140 (MNU35_04145)	886114..887619	+	1506	WP_145450233.1	PH domain-containing protein	-
MNU35_RS04145 (MNU35_04150)	887606..888124	+	519	WP_002447760.1	PH domain-containing protein	-
MNU35_RS04150 (MNU35_04155)	888163..888516	+	354	WP_002447759.1	holo-ACP synthase	-
MNU35_RS04155 (MNU35_04160)	888583..889731	+	1149	WP_119557509.1	alanine racemase	-
MNU35_RS04160 (MNU35_04165)	889818..889988	+	171	WP_001829931.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
MNU35_RS04165 (MNU35_04170)	889985..890347	+	363	WP_002447757.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MNU35_RS04170 (MNU35_04175)	890692..891693	+	1002	WP_002496969.1	PP2C family protein-serine/threonine phosphatase	-
MNU35_RS04175 (MNU35_04180)	891793..892119	+	327	WP_001829952.1	anti-sigma factor antagonist	-
MNU35_RS04180 (MNU35_04185)	892121..892600	+	480	WP_002447755.1	anti-sigma B factor RsbW	-
MNU35_RS04185 (MNU35_04190)	892575..893345	+	771	WP_002458689.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13483.62 Da
• Isoelectric Point: 9.9522

>T237743 WP_002447757.1 NZ_CP093170:889985-890347 [Staphylococcus epidermidis]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTFLSDSKMIEVDNALDISLGLNNFDHHKS

Nucleotide

• Length: 363 bp

>T237743 NZ_LR882500:c2226685-2226266 [Mycobacterium tuberculosis variant microti]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6289.92 Da
• Isoelectric Point: 4.1920

>AT237743 WP_001829931.1 NZ_CP093170:889818-889988 [Staphylococcus epidermidis]
MLSFNQNRNHSLEQSLKEGYAQMADLNLSLATEAFPIECEACDCNESHLISNSKNE

Nucleotide

• Length: 171 bp

>AT237743 NZ_LR882500:c2226954-2226694 [Mycobacterium tuberculosis variant microti]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0N1EF65