Detailed information of TA system TA023774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 2196284..2196863
• Replicon: chromosome
• Accession: NC_012632
• Organism: Sulfolobus islandicus M.16.27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C4KKM6
• Locus tag: M1627_RS11890
• Protein ID: WP_012719090.1
• Coordinates: 2196459..2196863 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C3N1J6
• Locus tag: M1627_RS11885
• Protein ID: WP_012719089.1
• Coordinates: 2196284..2196478 (+)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1627_RS11855 (M1627_2357)	2191633..2192508	+	876	WP_012712230.1	ABC transporter ATP-binding protein	-
M1627_RS11860 (M1627_2358)	2192499..2193116	+	618	WP_012712231.1	hypothetical protein	-
M1627_RS11865 (M1627_2359)	2193113..2193499	+	387	WP_009989099.1	hypothetical protein	-
M1627_RS11870 (M1627_2360)	2193496..2194206	-	711	WP_012712232.1	helix-turn-helix domain-containing protein	-
M1627_RS11875 (M1627_2361)	2194280..2195551	-	1272	WP_012712233.1	MFS transporter	-
M1627_RS11880 (M1627_2362)	2195606..2195893	-	288	WP_012719088.1	EamA family transporter	-
M1627_RS11885 (M1627_2364)	2196284..2196478	+	195	WP_012719089.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
M1627_RS11890 (M1627_2365)	2196459..2196863	+	405	WP_012719090.1	PIN domain-containing protein	Toxin
M1627_RS11895 (M1627_2366)	2197059..2197976	-	918	Protein_2382	IS110 family transposase	-
M1627_RS11900 (M1627_2367)	2198434..2199213	-	780	WP_016730261.1	DMT family transporter	-
M1627_RS11905	2200049..2200231	+	183	WP_009990866.1	hypothetical protein	-
M1627_RS11910 (M1627_2369)	2200379..2201140	+	762	WP_009990867.1	ZIP family metal transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-126)

Antitoxin

• SpoVT-AbrB domain (10-39)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15314.84 Da
• Isoelectric Point: 5.0349

>T23774 WP_012719090.1 NC_012632:2196459-2196863 [Sulfolobus islandicus M.16.27]
VEKAIVDTSVIIYDYVEDSEYHKRAEELLDSLDRWVIPVIVVHEIMSFLKGMKLEDKVDDVLAYSRSEKAEVVCDCTDNV
IDAIGVLKKEKLPLISYKNMVILSHAIREKLPLATFDRELSKIAKKYSVSLLQS

Nucleotide

• Length: 405 bp

>T23774 NC_012632:2196459-2196863 [Sulfolobus islandicus M.16.27]
GTGGAGAAGGCAATAGTTGACACTAGCGTGATAATTTACGACTACGTTGAAGACTCCGAATATCATAAGAGGGCTGAAGA
GCTTCTCGATAGTTTGGATAGGTGGGTAATTCCCGTGATTGTAGTTCATGAGATCATGTCGTTCTTAAAGGGCATGAAAC
TTGAAGACAAGGTAGATGACGTTTTGGCTTATAGTAGAAGTGAGAAAGCTGAGGTTGTGTGCGATTGTACAGATAACGTC
ATTGACGCTATAGGAGTGTTGAAGAAGGAAAAGCTTCCCTTGATCAGCTACAAAAATATGGTAATACTTTCTCACGCAAT
TAGAGAAAAATTGCCTTTAGCCACTTTTGATAGGGAACTCTCAAAGATAGCAAAGAAGTACTCCGTAAGTTTGCTACAGT
CGTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7152.20 Da
• Isoelectric Point: 8.6155

>AT23774 WP_012719089.1 NC_012632:2196284-2196478 [Sulfolobus islandicus M.16.27]
MIKSTPTSARSKINIKEGDILEVYLNGDEIVLRKVKTERPRIRLGNKLSLGDIEEAIERGEGNS

Nucleotide

• Length: 195 bp

>AT23774 NC_012632:2196284-2196478 [Sulfolobus islandicus M.16.27]
TTGATTAAGAGCACTCCAACCTCGGCTAGGAGTAAGATTAATATAAAAGAAGGAGATATACTTGAGGTTTACTTGAACGG
TGACGAGATAGTACTTCGGAAGGTTAAGACTGAGAGGCCGAGGATAAGGCTAGGCAATAAACTCTCACTGGGAGATATTG
AGGAGGCAATAGAGCGTGGAGAAGGCAATAGTTGA

Structures

Toxin

• AlphaFold DB: C4KKM6

Antitoxin

• AlphaFold DB: C3N1J6