Detailed information of TA system TA237736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4725345..4725961
• Replicon: chromosome
• Accession: NZ_CP093154
• Organism: Enterobacter asburiae strain 5549

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MND42_RS22900
• Protein ID: WP_080298642.1
• Coordinates: 4725345..4725716 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A808IJD0
• Locus tag: MND42_RS22905
• Protein ID: WP_023309753.1
• Coordinates: 4725719..4725961 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MND42_RS22885 (MND42_22885)	4722845..4723747	+	903	WP_008501831.1	formate dehydrogenase subunit beta	-
MND42_RS22890 (MND42_22890)	4723744..4724379	+	636	WP_023309750.1	formate dehydrogenase cytochrome b556 subunit	-
MND42_RS22895 (MND42_22895)	4724376..4725305	+	930	WP_045404233.1	formate dehydrogenase accessory protein FdhE	-
MND42_RS22900 (MND42_22900)	4725345..4725716	-	372	WP_080298642.1	type II toxin-antitoxin system VapC family toxin	Toxin
MND42_RS22905 (MND42_22905)	4725719..4725961	-	243	WP_023309753.1	ribbon-helix-helix protein, CopG family	Antitoxin
MND42_RS22910 (MND42_22910)	4726161..4727081	+	921	WP_045404232.1	alpha/beta hydrolase	-
MND42_RS22915 (MND42_22915)	4727090..4728031	-	942	WP_023309755.1	fatty acid biosynthesis protein FabY	-
MND42_RS22920 (MND42_22920)	4728076..4728513	-	438	WP_008501836.1	D-aminoacyl-tRNA deacylase	-
MND42_RS22925 (MND42_22925)	4728510..4729391	-	882	WP_023309756.1	virulence factor BrkB family protein	-
MND42_RS22930 (MND42_22930)	4729385..4729984	-	600	WP_045404231.1	glucose-1-phosphatase	-
MND42_RS22935 (MND42_22935)	4730105..4730905	-	801	WP_045404230.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-109)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13688.85 Da
• Isoelectric Point: 6.9789

>T237736 WP_080298642.1 NZ_CP093154:c4725716-4725345 [Enterobacter asburiae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRNCPLISRNTKDFSGITGVVSPYQL

Nucleotide

• Length: 372 bp

>T237736 NZ_LR882500:1695619-1695936 [Mycobacterium tuberculosis variant microti]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9429.56 Da
• Isoelectric Point: 4.2803

>AT237736 WP_023309753.1 NZ_CP093154:c4725961-4725719 [Enterobacter asburiae]
MSLSADLDMGRILIDLSDDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT237736 NZ_LR882500:1695320-1695622 [Mycobacterium tuberculosis variant microti]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A808IJD0