Detailed information of TA system TA023773

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 894767..895373
• Replicon: chromosome
• Accession: NC_012632
• Organism: Sulfolobus islandicus M.16.27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C3MWJ7
• Locus tag: M1627_RS04565
• Protein ID: WP_012710924.1
• Coordinates: 894981..895373 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: C4KFX1
• Locus tag: M1627_RS04560
• Protein ID: WP_012710923.1
• Coordinates: 894767..894991 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1627_RS04550 (M1627_0917)	891400..892164	+	765	WP_012710921.1	enoyl-CoA hydratase/isomerase family protein	-
M1627_RS04555 (M1627_0918)	892167..894434	+	2268	WP_012718660.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
M1627_RS04560 (M1627_0919)	894767..894991	+	225	WP_012710923.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
M1627_RS04565 (M1627_0920)	894981..895373	+	393	WP_012710924.1	type II toxin-antitoxin system VapC family toxin	Toxin
M1627_RS04575 (M1627_0921)	895652..896748	+	1097	Protein_933	ISNCY family transposase	-
M1627_RS04580 (M1627_0922)	897221..897709	+	489	WP_012710925.1	hypothetical protein	-
M1627_RS04585 (M1627_0923)	897711..898646	+	936	WP_012710926.1	type I-A CRISPR-associated protein Cas7/Csa2	-
M1627_RS04590 (M1627_0924)	898647..899438	+	792	WP_012710927.1	type I-A CRISPR-associated protein Cas5a	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (23-118)

Antitoxin

• Post-segregation antitoxin CcdA (5-54)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 15005.69 Da
• Isoelectric Point: 8.3342

>T23773 WP_012710924.1 NC_012632:894981-895373 [Sulfolobus islandicus M.16.27]
MKDKEFLFDASALYSLLDYVDKVDVKKIHILTLTFYEVGNVIWKEHYIHKKVKDPITLSILFHKLMRKLNVVEDPPLDGV
MRIAVERGLTYYDASYAYVAESLGLILVSNDKELIRKANAISLKDLIKSM

Nucleotide

• Length: 393 bp

>T23773 NC_012632:894981-895373 [Sulfolobus islandicus M.16.27]
ATGAAAGATAAGGAATTCCTATTCGATGCCTCAGCTTTATATTCGCTTTTAGATTACGTGGATAAGGTAGATGTTAAGAA
AATTCATATACTTACCCTAACTTTTTATGAGGTAGGTAACGTCATATGGAAGGAGCATTATATACACAAAAAGGTTAAAG
ATCCTATAACTCTTTCGATACTTTTCCATAAATTAATGCGAAAACTTAACGTAGTAGAGGATCCGCCTCTTGATGGGGTA
ATGAGAATTGCCGTAGAAAGGGGTTTAACTTATTATGACGCATCTTATGCGTATGTTGCTGAGTCTTTAGGGCTTATCCT
AGTGTCTAATGATAAAGAGCTGATAAGAAAAGCTAATGCCATTTCGTTAAAAGATTTGATAAAAAGTATGTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8796.18 Da
• Isoelectric Point: 5.3410

>AT23773 WP_012710923.1 NC_012632:894767-894991 [Sulfolobus islandicus M.16.27]
MSDVISVRVKKELKKKAEELGINIREVVEKALEEAIKEKEKEELKDIAMRIKELMRDVSENDWVRAVRESRDER

Nucleotide

• Length: 225 bp

>AT23773 NC_012632:894767-894991 [Sulfolobus islandicus M.16.27]
ATGTCTGACGTAATAAGCGTAAGAGTGAAGAAAGAGTTGAAGAAGAAGGCAGAGGAGTTGGGAATTAACATTAGGGAAGT
TGTAGAAAAAGCTTTAGAGGAAGCCATAAAGGAGAAGGAGAAGGAAGAGCTTAAGGATATAGCTATGAGAATTAAGGAAC
TAATGAGGGATGTAAGTGAAAATGATTGGGTAAGGGCTGTTAGGGAGAGTAGAGATGAAAGATAA

Structures

Toxin

• AlphaFold DB: C3MWJ7

Antitoxin

• AlphaFold DB: C4KFX1