Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 1342037..1342701 Replicon chromosome
Accession NZ_CP093154
Organism Enterobacter asburiae strain 5549

Toxin (Protein)


Gene name ykfI Uniprot ID A0A157QTP3
Locus tag MND42_RS06505 Protein ID WP_007869731.1
Coordinates 1342037..1342363 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A0M7FI99
Locus tag MND42_RS06510 Protein ID WP_007869729.1
Coordinates 1342384..1342701 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MND42_RS06465 (MND42_06465) 1337283..1337420 + 138 WP_162184392.1 hypothetical protein -
MND42_RS06470 (MND42_06470) 1338054..1338386 + 333 Protein_1253 type VI secretion system contractile sheath small subunit -
MND42_RS06475 (MND42_06475) 1338355..1338648 - 294 WP_241510723.1 OmpA family protein -
MND42_RS06480 (MND42_06480) 1339012..1339242 + 231 Protein_1255 ATP-binding protein -
MND42_RS06485 (MND42_06485) 1339300..1339428 + 129 WP_229218363.1 hypothetical protein -
MND42_RS06490 (MND42_06490) 1339421..1339873 + 453 WP_045402629.1 type VI secretion system baseplate subunit TssE -
MND42_RS06495 (MND42_06495) 1339888..1340358 + 471 WP_045402632.1 hypothetical protein -
MND42_RS06500 (MND42_06500) 1340462..1341793 + 1332 WP_045402634.1 VasL domain-containing protein -
MND42_RS06505 (MND42_06505) 1342037..1342363 - 327 WP_007869731.1 TA system toxin CbtA family protein Toxin
MND42_RS06510 (MND42_06510) 1342384..1342701 - 318 WP_007869729.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MND42_RS06515 (MND42_06515) 1342715..1342942 - 228 WP_013098172.1 DUF987 domain-containing protein -
MND42_RS06520 (MND42_06520) 1342951..1343427 - 477 WP_013098171.1 RadC family protein -
MND42_RS06525 (MND42_06525) 1343443..1343901 - 459 WP_013098170.1 antirestriction protein -
MND42_RS06530 (MND42_06530) 1344005..1344244 - 240 WP_007869725.1 DUF905 domain-containing protein -
MND42_RS06535 (MND42_06535) 1344268..1345089 - 822 WP_007869723.1 DUF932 domain-containing protein -
MND42_RS06540 (MND42_06540) 1345216..1345512 - 297 WP_015386464.1 hypothetical protein -
MND42_RS06545 (MND42_06545) 1345556..1345828 - 273 WP_007869719.1 hypothetical protein -
MND42_RS06550 (MND42_06550) 1345885..1346622 - 738 WP_013098169.1 DeoR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1339888..1369132 29244


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-106)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 12299.25 Da        Isoelectric Point: 8.9951

>T237723 WP_007869731.1 NZ_CP093154:c1342363-1342037 [Enterobacter asburiae]
MKTLPATTQRAAKPCLSPVAVWQMLLARLLEQHYGLTINDTPFSDEVVIQQHIDAGITLSDAVNFLVEKYELVRIDRKGF
RWQEQSPYLRAVDILRARQAIGLLRGSH

Download         Length: 327 bp

>T237723 NZ_LR882500:c755420-755037 [Mycobacterium tuberculosis variant microti]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11917.60 Da        Isoelectric Point: 7.0264

>AT237723 WP_007869729.1 NZ_CP093154:c1342701-1342384 [Enterobacter asburiae]
MTHIIWGLQRAITPRLGARLVQEGNRLHYLADRASITGMFSDAECRKLDDTFPHFIRQMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT237723 NZ_LR882500:c755670-755515 [Mycobacterium tuberculosis variant microti]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A157QTP3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M7FI99

References