Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 125797..126440 Replicon plasmid pCY814036-KPC2
Accession NZ_CP093153
Organism Klebsiella pneumoniae strain CY814036

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MMZ10_RS28660 Protein ID WP_001044770.1
Coordinates 126024..126440 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MMZ10_RS28655 Protein ID WP_001261282.1
Coordinates 125797..126027 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MMZ10_RS28620 (121020) 121020..121799 - 780 WP_013214009.1 site-specific integrase -
MMZ10_RS28625 (121983) 121983..122987 - 1005 WP_011977814.1 hypothetical protein -
MMZ10_RS28630 (123017) 123017..123220 - 204 WP_011977813.1 hypothetical protein -
MMZ10_RS28635 (123266) 123266..123787 - 522 WP_013214008.1 hypothetical protein -
MMZ10_RS28640 (123845) 123845..124237 - 393 WP_011977811.1 hypothetical protein -
MMZ10_RS28645 (124274) 124274..125218 - 945 WP_011977810.1 hypothetical protein -
MMZ10_RS28650 (125379) 125379..125840 - 462 WP_014343465.1 hypothetical protein -
MMZ10_RS28655 (125797) 125797..126027 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MMZ10_RS28660 (126024) 126024..126440 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MMZ10_RS28665 (126514) 126514..128076 + 1563 WP_004206609.1 AAA family ATPase -
MMZ10_RS28670 (128061) 128061..129083 + 1023 WP_000361404.1 helicase UvrD -
MMZ10_RS28675 (129339) 129339..130036 + 698 WP_223174630.1 IS1 family transposase -
MMZ10_RS28680 (130065) 130065..130337 + 273 Protein_171 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB - 1..140105 140105


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T237720 WP_001044770.1 NZ_CP093153:126024-126440 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T237720 NZ_LR882500:712525-712926 [Mycobacterium tuberculosis variant microti]
GTGACGGTGCTGCTCGACGCCAACGTGCTGATCGCATTGGTGGTCGCCGAGCATGTGCATCATGATGCCGCAGCGGACTG
GCTCATGGCGTCCGACACCGGATTTGCGACCTGCCCGATGACACAAGGAAGCCTGGTTCGATTCCTGGTGCGCTCGGGAC
AGTCCGCGGCGGCGGCTCGGGATGTCGTCAGTGCGGTCCAGTGCACGAGCCGCCACGAATTCTGGCCCGATGCACTCTCT
TTCGCCGGTGTCGAGGTCGCTGGTGTGGTTGGGCACCGGCAGGTGACCGATGCCTACCTTGCCCAGCTCGCGCGAAGCCA
CGACGGGCAGTTGGCGACGCTCGACAGCGGCTTAGCACACCTGCACGGCGACGTCGCGGTACTCATTCCAACGACCACCT
GA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT237720 WP_001261282.1 NZ_CP093153:125797-126027 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT237720 NZ_LR882500:712301-712528 [Mycobacterium tuberculosis variant microti]
GTGCGCACTACCATCGACCTGCCGCAAGACCTGCACAAGCAGGCACTGGCGATTGCCCGGGATACGCACCGCACGTTGAG
TGAAACGGTCGCCGACCTCATGCGACGAGGCCTGGTCGCCAACCGCCCTACCGCGTTGTCCTCAGACCCCAGAACGGGAT
TGCCTTTGGTGAGCGTCGGGACCGTCGTGACCTCCGAGGACGTGCGTTCATTAGAGGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References