Detailed information of TA system TA023772

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-AbrB
• Location: 816068..816671
• Replicon: chromosome
• Accession: NC_012632
• Organism: Sulfolobus islandicus M.16.27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C3N456
• Locus tag: M1627_RS04195
• Protein ID: WP_012718602.1
• Coordinates: 816282..816671 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M9UC56
• Locus tag: M1627_RS04190
• Protein ID: WP_012710994.1
• Coordinates: 816068..816295 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M1627_RS04165 (M1627_0845)	811582..812397	+	816	WP_012718598.1	N-acyl homoserine lactonase family protein	-
M1627_RS04170 (M1627_0846)	812404..813699	+	1296	WP_012718599.1	hydroxyacid-oxoacid transhydrogenase	-
M1627_RS04175 (M1627_0847)	813702..815126	-	1425	WP_012718600.1	phenylacetate--CoA ligase family protein	-
M1627_RS15685	815421..815799	-	379	Protein_860	PaREP1 family protein	-
M1627_RS04190 (M1627_0849)	816068..816295	+	228	WP_012710994.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
M1627_RS04195 (M1627_0850)	816282..816671	+	390	WP_012718602.1	PIN domain-containing protein	Toxin
M1627_RS04200 (M1627_0851)	816756..817160	+	405	WP_012718603.1	HEPN domain-containing protein	-
M1627_RS04205 (M1627_0852)	817145..817480	+	336	WP_009072970.1	nucleotidyltransferase domain-containing protein	-
M1627_RS15690 (M1627_0853)	817576..818652	-	1077	Protein_865	IS110 family transposase	-
M1627_RS04220 (M1627_0854)	819247..820470	+	1224	WP_012718605.1	NAD(P)/FAD-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	817846..818277	431

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• SpoVT-AbrB domain (3-47)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14828.43 Da
• Isoelectric Point: 4.8625

>T23772 WP_012718602.1 NC_012632:816282-816671 [Sulfolobus islandicus M.16.27]
MIVVDTNILVYSTFEDSENHSKALEIIEKEDVKIPQIVVYEFLWVLAKLTSDVSLIKTKIEELREFEIICENPETILNGV
KMLKEDGKPLKMLNDYVILALAKQLKGDLATYDEKLRKIAEKHGVRIIP

Nucleotide

• Length: 390 bp

>T23772 NC_012632:816282-816671 [Sulfolobus islandicus M.16.27]
ATGATAGTAGTTGACACGAACATATTAGTTTATTCAACTTTTGAAGATTCCGAAAACCATTCTAAAGCTCTAGAAATTAT
AGAAAAGGAGGACGTTAAAATTCCGCAGATAGTCGTTTATGAGTTCTTATGGGTTTTAGCTAAACTTACATCAGATGTTT
CCTTAATAAAAACTAAGATTGAAGAGTTAAGAGAGTTTGAAATTATATGCGAAAACCCGGAAACCATACTCAATGGTGTA
AAAATGTTAAAGGAAGACGGTAAACCTTTAAAGATGTTAAATGATTACGTTATTCTAGCCTTAGCTAAGCAATTAAAAGG
AGATTTAGCAACCTATGATGAAAAATTAAGGAAAATTGCTGAAAAACACGGTGTAAGAATAATTCCTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8668.92 Da
• Isoelectric Point: 5.1486

>AT23772 WP_012710994.1 NC_012632:816068-816295 [Sulfolobus islandicus M.16.27]
MKVKVTRNFQVTIPSEIREKLGIKEGDYVEVTLDESNGTMIIKPYIRKWTTIRLNKKVDQEDIDKAVEEALNDSS

Nucleotide

• Length: 228 bp

>AT23772 NC_012632:816068-816295 [Sulfolobus islandicus M.16.27]
ATGAAAGTAAAGGTTACTAGAAACTTTCAAGTTACTATACCCTCAGAGATAAGAGAAAAATTAGGTATAAAGGAAGGAGA
TTATGTCGAAGTAACTTTGGATGAAAGTAATGGAACTATGATAATTAAGCCGTATATAAGAAAGTGGACTACAATAAGGT
TAAATAAAAAAGTAGACCAAGAGGATATAGATAAGGCAGTTGAGGAGGCACTAAATGATAGTAGTTGA

Structures

Toxin

• AlphaFold DB: C3N456

Antitoxin

• AlphaFold DB: M9UC56