Detailed information of TA system TA237716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 100903..101639
• Replicon: plasmid pCY814036-iucA
• Accession: NZ_CP093152
• Organism: Klebsiella pneumoniae strain CY814036

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: MMZ10_RS26970
• Protein ID: WP_004187044.1
• Coordinates: 101157..101639 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: MMZ10_RS26965
• Protein ID: WP_003026799.1
• Coordinates: 100903..101169 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MMZ10_RS26950 (MMZ10_26950)	96375..97769	+	1395	WP_032439652.1	cytosine permease	-
MMZ10_RS26955 (MMZ10_26955)	97781..98989	+	1209	WP_032448288.1	imidazolonepropionase	-
MMZ10_RS26960 (MMZ10_26960)	98982..99791	+	810	WP_023329017.1	N-formylglutamate deformylase	-
MMZ10_RS26965 (MMZ10_26965)	100903..101169	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
MMZ10_RS26970 (MMZ10_26970)	101157..101639	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
MMZ10_RS26975 (MMZ10_26975)	101850..103196	+	1347	WP_077251107.1	ISNCY family transposase	-
MMZ10_RS26980 (MMZ10_26980)	103356..104060	+	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
MMZ10_RS26985 (MMZ10_26985)	104337..105683	+	1347	WP_077254728.1	ISNCY family transposase	-
MMZ10_RS26990 (MMZ10_26990)	105732..106130	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
MMZ10_RS26995 (MMZ10_26995)	106278..106388	-	111	Protein_106	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / dfrA14 / ant(3'')-Ia / blaOXA-10 / cmlA1 / ARR-3 / blaLAP-2 / qnrS1	iutA / iucD / iucC / iucB / iucA	1..257343	257343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T237716 WP_004187044.1 NZ_CP093152:101157-101639 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T237716 NZ_LR882500:679431-679838 [Mycobacterium tuberculosis variant microti]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT237716 WP_003026799.1 NZ_CP093152:100903-101169 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT237716 NZ_LR882500:679219-679434 [Mycobacterium tuberculosis variant microti]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66